Autoantibody-induced internalization of nicotinic acetylcholine receptor α3 subunit exogenously expressed in human embryonic kidney cells

被引：14

作者：

Kobayashi, Shota ^{[1
,2
]}

Yokoyama, Shigeru ^{[3
]}

Maruta, Takahiro ^{[4
]}

Negami, Masako ^{[5
]}

Muroyama, Akiko ^{[1
]}

Mitsumoto, Yasuhide ^{[1
]}

Iwasa, Kazuo ^{[6
]}

Yamada, Masahito ^{[6
]}

Yoshikawa, Hiroaki ^{[2
,6
]}

机构：

[1] Hokuriku Univ, Lab Alternat Med & Expt Therapeut, Dept Clin Pharm, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9201181, Japan

[2] Kanazawa Univ, Hlth Serv Ctr, Kanazawa, Ishikawa 9201192, Japan

[3] Kanazawa Univ, Grad Sch Med, Dept Biophys Genet, Kanazawa, Ishikawa 9208640, Japan

[4] Kanazawa Nishi Hosp, Neurol Ctr, Kanazawa, Ishikawa 9200025, Japan

[5] Keiju Med Ctr, Nanao, Ishikawa 9268605, Japan

[6] Kanazawa Univ, Grad Sch Med Sci, Dept Neurol & Neurobiol Aging, Kanazawa, Ishikawa 9200025, Japan

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2013年 / 257卷 / 1-2期

关键词：

Nicotinic acetylcholine receptor alpha 3 subunit; Autoantibody; Receptor internalization; AUTONOMIC NEUROPATHY; NEURONS;

D O I：

10.1016/j.jneuroim.2012.12.010

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Autoantibody against nicotinic acetylcholine receptor (nAChR) alpha 3 subunit has been implicated in the pathogenesis of paraneoplastic neurological syndrome. To examine the effect of anti-alpha 3 subunit autoantibody on cell-surface nAChRs, we established human embryonic kidney 293 cells stably co-expressing alpha 3 and beta 4 subunits. Upon incubation with seropositive patient's serum, this cell line showed co-accumulation of patient's IgG and alpha 3 subunits in the cytoplasm. These data support the hypothesis that anti-alpha 3 subunit autoantibody induces internalization of cell-surface nAChRs and thereby impairs synaptic transmission. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：102 / 106

页数：5

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