Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide

被引：84
作者：
Di Fiore, Anna [1 ]
Monti, Simona Maria [1 ]
Hilvo, Mika [2 ,3 ,4 ]
Parkkila, Seppo [2 ,3 ,4 ]
Romano, Vincenza [1 ]
Scaloni, Andrea [5 ]
Pedone, Carlo [1 ]
Scozzafava, Andrea [6 ]
Supuran, Claudiu T. [6 ]
De Simone, Giuseppina [1 ]
机构：
[1] CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy
[2] Univ Tampere, Inst Med Technol, FIN-33101 Tampere, Finland
[3] Univ Tampere, Sch Med, FIN-33101 Tampere, Finland
[4] Univ Tampere, Tampere Univ Hosp, FIN-33101 Tampere, Finland
[5] CNR, ISPAAM, Lab Prote & Spettrometria Massa, I-80147 Naples, Italy
[6] Univ Florence, Chim Bioorgan Lab, I-50019 Florence, Italy
来源：
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 2009年 / 74卷 / 01期
关键词：
crystal structure; human carbonic anhydrase XIII; inhibitors.protein-inhibitor complex; rational drug design; ISOZYME-II; X-RAY; PROTON-TRANSFER; THERAPEUTIC APPLICATIONS; ANTITUMOR SULFONAMIDE; EXTRACELLULAR DOMAIN; REFINED STRUCTURE; BINDING-SITE; CA-XIII; MEMBRANE;
D O I：
10.1002/prot.22144
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cytosolic isoform XIII is a recently discovered member of the human carbonic anhydrase (hCA, EC 4.2.1.1) family. It is selectively expressed among other tissues in the reproductive organs, where it may control pH and ion balance regulation, ensuring thus proper fertilization conditions. The authors report here the X-ray crystallographic structure of this isozyme in the unbound state and in complex with a classical sulfonamide inhibitor, namely acetazolamide. A detailed comparison of the obtained structural data with those already reported for other CA isozymes provides novel insights into the catalytic properties of the members of this protein family. On the basis of the inhibitory properties of acetazolamide against various cytosolic/transmembrane isoforms and the structural differences detected within the active site of the various CA isoforms, further prospects for the design of isozyme-specific CA inhibitors are here proposed.
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收藏
页码：164 / 175
页数：12
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