Metagenomics of bolidophyceae in plankton and ice of the White Sea

被引:9
作者
Belevich, T. A. [1 ]
Ilyash, L. V. [1 ]
Milyutina, I. A. [2 ]
Logacheva, M. D. [2 ,3 ]
Troitsky, A. V. [2 ]
机构
[1] Lomonosov Moscow State Univ, Fac Biol, Moscow 119991, Russia
[2] Lomonosov Moscow State Univ, Belozersky Inst Physicochem Biol, Moscow 119991, Russia
[3] Kazan Fed Univ, Inst Fundamental Biol & Med, Lab Extreme Biol, Kazan 420012, Russia
基金
俄罗斯科学基金会; 俄罗斯基础研究基金会;
关键词
metagenomic analysis; picophytoplankton; White Sea; Bolidophyceae; Triparma pacifica; Triparma strigata; OYASHIO REGION; COMMUNITY STRUCTURE; DIVERSITY; PARMALES; ABUNDANCE; PICOEUKARYOTES; NANOPLANKTON; SUMMER; GROWTH;
D O I
10.1134/S0006297917120136
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The molecular diversity of poorly studied algae of Bolidophyceae class was first estimated by Illumina sequencing of V4 region of 18S rRNA gene in ice, under-ice water and summer water of the subarctic White Sea. We used two clustering thresholds-93 and 97%-and revealed 31 phylotypes of Bolidophyceae. Triparma pacifica and D cent. strigata were identified to species level. The association of individual phylotypes to certain biotopes (ice or plankton) and stages of seasonal succession (under ice or summer plankton) has been established. Some phylotypes are found in different biotopes and over a wide temperature range. Due to changing their genetic composition, Bolidophyceae are a constant component of the photoautotrophic plankton and ice communities.
引用
收藏
页码:1538 / 1548
页数:11
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