Recombinant interferons beta and gamma have a higher antiviral activity than interferon-alpha in coxsackievirus B3-infected carrier state cultures of human myocardial fibroblasts

被引：27

作者：

Heim, A ^{[1
]}

StilleSiegener, M ^{[1
]}

PringAkerblom, P ^{[1
]}

Grumbach, I ^{[1
]}

Brehm, C ^{[1
]}

Kreuzer, H ^{[1
]}

Figulla, HR ^{[1
]}

机构：

[1] UNIV GOTTINGEN,ABT KARDIOL,D-37075 GOTTINGEN,GERMANY

来源：

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH | 1996年 / 16卷 / 04期

关键词：

D O I：

10.1089/jir.1996.16.283

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We compared the antiviral activities of three recombinant human interferons (IFN-alpha 2a, IFN-beta, and IFN-gamma) in cultured human myocardial fibroblasts to select a candidate for trial in heart disease induced by cardiotropic enterovirus, e.g., coxsackievirus B3 (CVB3). Cells were exposed to CVB3, and after 7 days, when a persistent infection had developed, IFN was added. Virus yields were measured on alternate days for the next 7 or 16 days, and IFN activity was assessed as the percentage reduction in yield. IFN-gamma and IFN-beta were both highly active and reduced virus yields by 2 log (EC(99)) at concentrations of 23.4 IU/ml (SD = 8.6) and 10.1 IU/ml (SD = 3.2), respectively; with 250 IU/ml of either IFN, no infectious virus was formed. Unexpectedly, IFN-alpha 2a (EC(99) > 1250 IU/ml) was at least 120 times less active than IFN-beta; after use for 8 days or more, the minor effects it produced were no longer related to the concentration applied. Despite the pharmacokinetic advantages of IFN-alpha 2a, our data suggest that IFN-beta should in preference be evaluated in the clinic.

引用

页码：283 / 287

页数：5

共 41 条

[1] DIFFERENTIAL TYROSINE PHOSPHORYLATION OF THE IFNAR CHAIN OF THE TYPE-I INTERFERON RECEPTOR AND OF AN ASSOCIATED SURFACE PROTEIN IN RESPONSE TO IFN-ALPHA AND IFN-BETA [J].