Increased Expression and Aberrant Localization of Mucin 13 in Metastatic Colon Cancer

被引：42
作者：
Gupta, Brij K. [1 ]
Maher, Diane M. [1 ]
Ebeling, Mara C. [1 ]
Sundram, Vasudha [1 ]
Koch, Michael D.
Lynch, Douglas W.
Bohlmeyer, Teresa [1 ]
Watanabe, Akira [3 ,4 ]
Aburatani, Hiroyuki [3 ,4 ]
Puumala, Susan E. [2 ]
Jaggi, Meena [1 ]
Chauhan, Subhash C. [1 ]
机构：
[1] Sanford Res, Canc Biol Res Ctr, Sioux Falls, SD 57104 USA
[2] Sanford Res, Methodol & Data Anal Ctr, Sioux Falls, SD 57104 USA
[3] Univ S Dakota, Sioux Falls, SD USA
[4] Univ Tokyo, Genome Sci Div, Tokyo, Japan
来源：
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2012年 / 60卷 / 11期
关键词：
mucin; MUC13; colon cancer metastasis; immunohistochemistry; CELL-SURFACE MUCIN; MUC13; TUMORIGENESIS;
D O I：
10.1369/0022155412460678
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
MUC13 is a newly identified transmembrane mucin. Although MUC13 is known to be overexpressed in ovarian and gastric cancers, limited information is available regarding the expression of MUC13 in metastatic colon cancer. Herein, we investigated the expression profile of MUC13 in colon cancer using a novel anti-MUC13 monoclonal antibody (MAb, clone ppz0020) by immunohistochemical (IHC) analysis. A cohort of colon cancer samples and tissue microarrays containing adjacent normal, non-metastatic colon cancer, metastatic colon cancer, and liver metastasis tissues was used in this study to investigate the expression pattern of MUC13. IHC analysis revealed significantly higher (p < 0.001) MUC13 expression in non-metastatic colon cancer samples compared with faint or very low expression in adjacent normal tissues. Interestingly, metastatic colon cancer and liver metastasis tissue samples demonstrated significantly (p < 0.05) higher cytoplasmic and nuclear MUC13 expression compared with non-metastatic colon cancer and adjacent normal colon samples. Moreover, cytoplasmic and nuclear MUC13 expression correlated with larger and poorly differentiated tumors. Four of six tested colon cancer cell lines also expressed MUC13 at RNA and protein levels. These studies demonstrate a significant increase in MUC13 expression in metastatic colon cancer and suggest a correlation between aberrant MUC13 localization (cytoplasmic and nuclear expression) and metastatic colon cancer. (J Histochem Cytochem 60:822-831, 2012)
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页码：822 / 831
页数：10
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