Rapid continuous colorimetric enzyme assay for penicillin G acylase

被引：7
|
作者：
Simons, H [1 ]
Gibson, TD [1 ]
机构：
[1] Appl Enzyme Technol Ltd, Leeds LS2 3AR, W Yorkshire, England
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 06期
关键词：
colorimetric assay; penicillin G acylase; Phenol Red;
D O I：
10.1023/A:1008975600792
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid, continuous, colorimetric enzyme assay for penicillin G acylase has been developed. The assay measures the formation of the acidic products of penicillin G hydrolysis by following the decrease in pH using Phenol Red as an indicator. The activity measured is directly proportional to the amount of enzyme added to the assay, having a linear relationship with an R-2 value of 0.9994.
引用
收藏
页码：365 / 367
页数：3
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