Cloning, expression, and chromosomal localization of the rat mitochondrial capsule selenoprotein gene (MCS), the reading frame does not contain potential UGA selenocysteine codons

被引：19

作者：

Adham, IM ^{[1
]}

Tessmann, D ^{[1
]}

Soliman, KA ^{[1
]}

Murphy, D ^{[1
]}

Kremling, H ^{[1
]}

Szpirer, C ^{[1
]}

Engel, W ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS,DEPT BIOL MOLEC,BRUSSELS,BELGIUM

来源：

DNA AND CELL BIOLOGY | 1996年 / 15卷 / 02期

关键词：

D O I：

10.1089/dna.1996.15.159

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitochondrial capsule selenoprotein (MCS) is a selenium-containing polypeptide. It is one of three proteins that are important for the maintenance and stabilization of the crescent structure of the sperm mitochondria. In this paper, we report the isolation and characterization of the rat MCS cDNA and gene. The cDNA contains a reading frame for a 145-amino-acid protein and it lacks the UGA codons, which have been found in the reading frame of the mouse MCS cDNA and have been presumed to encode the selenocysteine in the amino terminal of the deduced mouse amino acid sequence. The deduced amino acid sequence of the rat and mouse MCS shows a high level of homology (79%). The rat MCS gene contains two exons; the intron sequence interrupts the 5' untranslated sequence at the same position as in the mouse MCS gene. The transcription start site is located 184 bp upstream of the translation start site. Alignment of the 5'-flanking regions of the mouse and rat genes reveals that the first 400 nucleotides upstream of the transcription start site exhibit an overall sequence similarity of 73%. This conserved region contains no TATA or CAAT box motifs. Northern blot analysis indicates that the MCS mRNA is detectable only in the testis after day 30 of postnatal development. Moreover, in situ hybridization revealed that the rat MCS gene is mainly expressed in round spermatids. From the analysis of mouse-rat cell hybrids that segregate rat chromosomes, the MCS gene was assigned to rat chromosome 2.

引用

页码：159 / 166

页数：8

共 33 条

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