Segregation of rol Genes in Two Generations of Sinningia speciosa Engineered Through Wild Type Rhizobium rhizogenes

被引：15

作者：

Desmet, Siel ^{[1
,2
]}

Dhooghe, Emmy ^{[1
]}

De Keyser, Ellen ^{[1
]}

Quataert, Paul ^{[1
]}

Eeckhaut, Tom ^{[1
]}

Van Huylenbroeck, Johan ^{[1
]}

Geelen, Danny ^{[2
]}

机构：

[1] Flanders Res Inst Agr Fisheries & Food Res, Plant Sci Unit, Melle, Belgium

[2] Univ Ghent, Fac Biosci Engn, Dept Plants & Crops, Ghent, Belgium

来源：

FRONTIERS IN PLANT SCIENCE | 2020年 / 11卷

关键词：

Rhizobium rhizogenes; Ri phenotype; hairy root; root inducing plasmid; ddPCR; rolgenes; florist's gloxinia; RAPE BRASSICA-NAPUS; RI T-DNA; AGROBACTERIUM-RHIZOGENES; MEDIATED TRANSFORMATION; PLANT-REGENERATION; TRANSGENIC PLANTS; ROOT CULTURES; PCR PRIMERS; PLASMID; GROWTH;

D O I：

10.3389/fpls.2020.00859

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Rhizobium rhizogenesinfects and transforms a wide range of plant species. It thereby introduces new genes located on transfer-DNA of the root inducing plasmid (pRi) into the plant genome and one of its abilities is to alter the host root system. Explants from pRi transformed roots fromSinningia speciosawere regenerated to create naturally transgenic Ri lines. The presence ofrolandauxgenes in the Ri lines was linked with altered growth characteristics: shorter peduncles, wrinkled leaves, delayed flowering and enhanced root growth. The potential of Ri lines for breeding was evaluated through consecutive backcrossing with the original host genotype. The progeny of reciprocal crosses showed non-Mendelian inheritance suggesting partial transmission of the of theauxandrolgenes. The typical Ri phenotype observed in the primary Ri line was partially inherited. These results revealed that the Ri phenotype is a complex trait influenced by the genetic background of the Ri line.

引用

页数：18

共 81 条

[1]

Acquaah G., 2012, Principles of plant genetics and breeding, VSecond, P385, DOI DOI 10.1002/9781118313718

[2]

[Anonymous], 2019, A Language and Environment for Statistical Computing

[3] Bioactive constituent production in St. John's Wort in vitro hairy roots. regenerated plant lines [J].