Genome-wide linkage mapping of QTL for black point reaction in bread wheat (Triticum aestivum L.)

被引：34

作者：

Liu, Jindong ^{[1
,2
]}

He, Zhonghu ^{[1
,5
]}

Wu, Ling ^{[3
]}

Bai, Bin ^{[4
]}

Wen, Weie ^{[1
]}

Xie, Chaojie ^{[2
]}

Xia, Xianchun ^{[1
]}

机构：

[1] CAAS, Inst Crop Sci, Natl Wheat Improvement Ctr, 12 Zhongguancun South St, Beijing 100081, Peoples R China

[2] China Agr Univ, Dept Plant Genet & Breeding, State Key Lab Agrobiotechnol, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China

[3] Sichuan Acad Agr Sci, Crop Res Inst, 20 Jingjusi Rd, Chengdu 610066, Sichuan, Peoples R China

[4] Gansu Acad Agr Sci, Wheat Res Inst, Lanzhou 730070, Gansu, Peoples R China

[5] CAAS, Int Maize & Wheat Improvement Ctr CIMMYT, China Off, 12 Zhongguancun South St, Beijing 100081, Peoples R China

来源：

THEORETICAL AND APPLIED GENETICS | 2016年 / 129卷 / 11期

基金：

中国国家自然科学基金;

关键词：

POLYPHENOL OXIDASE ACTIVITY; QUANTITATIVE TRAIT LOCI; DURUM-WHEAT; FUMONISIN CONTAMINATION; ALTERNARIA-INFECTORIA; CANDIDATE GENES; PLANT-GROWTH; MAP; RESISTANCE; MARKERS;

D O I：

10.1007/s00122-016-2766-3

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Nine QTL for black point resistance in wheat were identified using a RIL population derived from a Linmai 2/Zhong 892 cross and 90K SNP assay. Black point, discoloration of the embryo end of the grain, downgrades wheat grain quality leading to significant economic losses to the wheat industry. The availability of molecular markers will accelerate improvement of black point resistance in wheat breeding. The aims of this study were to identify quantitative trait loci (QTL) for black point resistance and tightly linked molecular markers, and to search for candidate genes using a high-density genetic linkage map of wheat. A recombinant inbred line (RIL) population derived from the cross Linmai 2/Zhong 892 was evaluated for black point reaction during the 2011-2012, 2012-2013 and 2013-2014 cropping seasons, providing data for seven environments. A high-density linkage map was constructed by genotyping the RILs with the wheat 90K single nucleotide polymorphism (SNP) chip. Composite interval mapping detected nine QTL on chromosomes 2AL, 2BL, 3AL, 3BL, 5AS, 6A, 7AL (2) and 7BS, designated as QBp.caas-2AL, QBp.caas-2BL, QBp.caas-3AL, QBp.caas-3BL, QBp.caas-5AS, QBp.caas-6A, QBp.caas-7AL.1, QBp.caas-7AL.2 and QBp.caas-7BS, respectively. All resistance alleles, except for QBp.caas-7AL.1 from Linmai 2, were contributed by Zhong 892. QBp.caas-3BL, QBp.caas-5AS, QBp.caas-7AL.1, QBp.caas-7AL.2 and QBp.caas-7BS probably represent new loci for black point resistance. Sequences of tightly linked SNPs were used to survey wheat and related cereal genomes identifying three candidate genes for black point resistance. The tightly linked SNP markers can be used in marker-assisted breeding in combination with the kompetitive allele specific PCR technique to improve black point resistance.

引用

页码：2179 / 2190

页数：12

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