Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro

被引：12

作者：

Xu Qinqin ^{[1
]}

Liu Zhenhua ^{[1
]}

Sun Yisheng ^{[1
]}

Ding Zhongjie ^{[1
]}

Lu Longxian ^{[1
]}

Li Yongquan ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CHINESE JOURNAL OF CHEMICAL ENGINEERING | 2012年 / 20卷 / 02期

基金：

国家高技术研究发展计划(863计划);

关键词：

antioxidant activity; Cordyceps ophioglossoides L2; intracellular polysaccharide; submerged culture; optimization; PROTEIN-BOUND POLYSACCHARIDE; HOT-WATER EXTRACTS; ANTIMICROBIAL ACTIVITIES; ANTITUMOR-ACTIVITY; MYCELIAL GROWTH; SINENSIS; REQUIREMENTS;

D O I：

10.1016/S1004-9541(12)60391-7

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Cordyceps ophioglossoides is a valuable traditional medicinal material. We have found that intracellular polysaccharide (IPS) is the major biologically active ingredient in Cordyceps ophioglossoides. This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides. The optimal medium for IPS production consists of glucose 54.50 g.L-1, yeast powder 25.50 g.L-1, NaH2PO4 0.4 g.L-1 and K2HPO4 0.4 g.L-1. The suggested culture conditions are 24 degrees C, initial pH 4.5 with a rotary speed of 120 r.min(-1) for 168 h. The yield of IPS is 737.93 mg.L-1, which is 50% higher than the yield under the conditions prior to optimization. The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay. The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative diseases and menopausal symptoms.

引用

页码：294 / 301

页数：8