Droplet-Array (DA) Sandwich Chip: A Versatile Platform for High-Throughput Cell Screening Based on Superhydrophobic-Superhydrophilic Micropatterning

被引：181

作者：

Popova, Anna A. ^{[1
]}

Schillo, Sebastian M. ^{[2
]}

Demir, Konstantin ^{[1
]}

Ueda, Erica ^{[1
]}

Nesterov-Mueller, A. ^{[2
]}

Levkin, Pavel A. ^{[1
]}

机构：

[1] Karlsruhe Inst Technol, Inst Toxicol & Genet, D-76344 Eggenstein Leopoldshafen, Germany

[2] Karlsruhe Inst Technol, Inst Microstruct Technol, D-76344 Eggenstein Leopoldshafen, Germany

来源：

ADVANCED MATERIALS | 2015年 / 27卷 / 35期

关键词：

droplet-array sandwich chips; high throughput screening; superhydrophobic-superhydrophilic micropatterning; MICROARRAYS; ASSAY; CULTURE; FACILE;

D O I：

10.1002/adma.201502115

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A droplet-array (DA) sandwich chip is a miniaturized platform for cell-based high-throughput screening. It is based on sandwiching of a glass slide with a preprinted library and a superhydrophobic-superhydrophilic pattern, which consists of thousands of simultaneously formed microdroplets containing cells. The DA sandwich chip allows for one-step cell seeding, simultaneous initiation of screening, and 1000 times less reagent consumption than a regular 96-well plate. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：5217 / 5222

页数：6

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