Profiling differences in chemical composition of brain structures using Raman spectroscopy

被引：15

作者：

Dakovic, Marko ^{[1
]}

Stojiljkovic, Aleksandra S. ^{[1
]}

Bajuk-Bogdanovic, Danica ^{[1
]}

Starcevic, Ana ^{[2
]}

Puskas, Laslo ^{[2
]}

Filipovic, Branislav ^{[2
]}

Uskokovic-Markovic, Snezana ^{[3
]}

Holclajtner-Antunovic, Ivanka ^{[1
]}

机构：

[1] Univ Belgrade, Fac Phys Chem, Belgrade, Serbia

[2] Univ Belgrade, Sch Med, Inst Anat Niko Miljanic, Belgrade, Serbia

[3] Univ Belgrade, Fac Pharm, Belgrade, Serbia

来源：

TALANTA | 2013年 / 117卷

关键词：

Raman spectroscopy; Brain tissue; Chemical composition; Independent component analysis; INDEPENDENT COMPONENT ANALYSIS; RAT-BRAIN; INTRACRANIAL TUMORS; TISSUE; ALGORITHMS; SPECTRA; CELLS;

D O I：

10.1016/j.talanta.2013.08.058

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Raman spectroscopy enables non-invasive investigation of chemical composition of biological tissues. Due to similar chemical composition, the analysis of Raman spectra of brain structures and assignment of their spectral features to chemical constituents presents a particular challenge. In this study we demonstrate that standard and independent component analysis of Raman spectra is capable of assessment of differences in chemical composition between functionally related gray and white matter structures. Our results show the ability of Raman spectroscopy to successfully depict variation in chemical composition between structurally similar and/or functionally connected brain structures. The observed differences were attributed to variations in content of proteins and lipids in these structures. Independent component analysis enabled separation of contributions of major constituents in spectra and revealed spectral signatures of low-concentration metabolites. This provided finding of discrepancies between structures of striatum as well as between white matter structures. Raman spectroscopy can provide information about variations in contents of major chemical constituents in brain structures, while the application of independent component analysis performed on obtained spectra can help in revealing minute differences between closely related brain structures. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：133 / 138

页数：6

共 35 条

[1] Discriminating healthy from tumor and necrosis tissue in rat brain tissue samples by Raman spectral imaging [J].