PEGylated Phospholipid Micelle-Encapsulated Near-Infrared PbS Quantum Dots for in vitro and in vivo Bioimaging

被引：63

作者：

Hu, Rui ^{[1
,2
]}

Law, Wing-Cheung ^{[2
]}

Lin, Guimiao ^{[1
]}

Ye, Ling ^{[3
]}

Liu, Jianwei ^{[3
]}

Liu, Jing ^{[3
]}

Reynolds, Jessica L. ^{[4
]}

Yong, Ken-Tye ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Elect & Elect Engn, Singapore 639798, Singapore

[2] SUNY Buffalo, Inst Lasers Photon & Biophoton, Buffalo, NY 14260 USA

[3] Chinese Peoples Liberat Army Gen Hosp, Inst Gerontol & Geriatr, Beijing 100853, Peoples R China

[4] SUNY Buffalo, Innovat Ctr, Dept Med, Div Allergy Immunol & Rheumatol, Buffalo, NY 14203 USA

来源：

THERANOSTICS | 2012年 / 2卷 / 07期

关键词：

Phospholipid; Micelle encapsulation; quantum dots; bioimaging; PEG; TETRAZOLIUM FORMAZAN ASSAY; FLUORESCENT PROTEIN; NANOCRYSTALS; DELIVERY; BILAYERS; THERAPY; FOLATE; AGENTS; DRUGS; LIGHT;

D O I：

10.7150/thno.4275

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Surface modification and functionalization of bioconjugated quantum dots (QDs) has drawn great attention for the past few years due to their wide applications in biomedical research. In this contribution, we demonstrate the use of PEGylated phospholipid micelles to encapsulate near infrared emitting ultra-small lead sulfide (PbS) QDs for in vitro and in vivo imaging. The cytotoxicity of the micelle-encapsulated QDs formulation was evaluated using MTS assay and histological analysis studies. We have found that upon encapsulating the QDs with phospholipid micelle, the toxicity of the PbS QDs is reduced, from which we envision that the PEGylated phospholipid micelle-encapsulated PbS QDs formulation can be used as theranostics probes for some selected applications in cell imaging and small animals study.

引用

页码：723 / 733

页数：11

共 45 条

[1] Semiconductor clusters, nanocrystals, and quantum dots [J].

Alivisatos, AP .

SCIENCE, 1996, 271 (5251) :933-937

[2] In vitro characterization of PEGylated phospholipid micelles for improved drug solubilization:: Effects of PEG chain length and PC incorporation [J].

Ashok, B ;

Arleth, L ;

Hjelm, RP ;

Rubinstein, I ;

Önyüksel, H .

JOURNAL OF PHARMACEUTICAL SCIENCES, 2004, 93 (10) :2476-2487

[3] NEGATIVE STAINING OF PHOSPHOLIPIDS + THEIR STRUCTURAL MODIFICATION BY-SURFACE ACTIVE AGENTS AS OBSERVED IN ELECTRON MICROSCOPE [J].

BANGHAM, AD ;

HORNE, RW .

JOURNAL OF MOLECULAR BIOLOGY, 1964, 8 (05) :660-&

[4] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[5] USE OF AN AQUEOUS SOLUBLE TETRAZOLIUM FORMAZAN ASSAY TO MEASURE VIABILITY AND PROLIFERATION OF LYMPHOKINE-DEPENDENT CELL-LINES [J].

BUTTKE, TM ;

MCCUBREY, JA ;

OWEN, TC .

JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 157 (1-2) :233-240

[6] Peptide-labeled near-infrared quantum dots for imaging tumor vasculature in living subjects [J].

Cai, WB ;

Shin, DW ;

Chen, K ;

Gheysens, O ;

Cao, QZ ;

Wang, SX ;

Gambhir, SS ;

Chen, XY .

NANO LETTERS, 2006, 6 (04) :669-676

[7] Renal clearance of quantum dots [J].

Choi, Hak Soo ;

Liu, Wenhao ;

Misra, Preeti ;

Tanaka, Eiichi ;

Zimmer, John P. ;

Ipe, Binil Itty ;

Bawendi, Moungi G. ;

Frangioni, John V. .

NATURE BIOTECHNOLOGY, 2007, 25 (10) :1165-1170

[8] Tissue- and Organ-Selective Biodistribution of NIR Fluorescent Quantum Dots [J].

Choi, Hak Soo ;

Ipe, Binil Itty ;

Misra, Preeti ;

Lee, Jeong Heon ;

Bawendi, Moungi G. ;

Frangioni, John V. .

NANO LETTERS, 2009, 9 (06) :2354-2359

[9] USE OF AN AQUEOUS SOLUBLE TETRAZOLIUM FORMAZAN ASSAY FOR CELL-GROWTH ASSAYS IN CULTURE [J].

CORY, AH ;

OWEN, TC ;

BARLTROP, JA ;

CORY, JG .

CANCER COMMUNICATIONS, 1991, 3 (07) :207-212

[10] In vivo imaging of quantum dots encapsulated in phospholipid micelles [J].

Dubertret, B ;

Skourides, P ;

Norris, DJ ;

Noireaux, V ;

Brivanlou, AH ;

Libchaber, A .

SCIENCE, 2002, 298 (5599) :1759-1762

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