DNA-binding, antioxidant activity, and bioactivity studies of ruthenium(II) complexes containing amino substituents

被引：9

作者：

Xie, Yang-Yin ^{[1
]}

Jiang, Guang-Bin ^{[1
]}

Yao, Jun-Hua ^{[2
]}

Lin, Gan-Jian ^{[1
]}

Huang, Hong-Liang ^{[3
]}

Wang, Xiu-Zhen ^{[1
]}

Liu, Yun-Jun ^{[1
]}

机构：

[1] Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Instrument Anal & Res Ctr, Guangzhou 510275, Guangdong, Peoples R China

[3] Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou, Guangdong, Peoples R China

来源：

JOURNAL OF COORDINATION CHEMISTRY | 2013年 / 66卷 / 14期

关键词：

Ruthenium(II) complexes; DNA-binding; Antioxidant activity; Bioactivity; CYTOTOXICITY IN-VITRO; CELL-CYCLE ARREST; POLYPYRIDYL COMPLEXES; LIGHT SWITCH; PHOTOCLEAVAGE; APOPTOSIS; DIPYRIDOPHENAZINE; PORPHYRINS; OXIDATION; WATER;

D O I：

10.1080/00958972.2013.805214

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Two new Ru(II) complexes, [Ru(dmp)(2)(dadppz)](ClO4)(2) (1) and [Ru(dip)(2)(dadppz)](ClO4)(2) (2), were synthesized and characterized by elemental analysis, ES-MS, and H-1 NMR. DNA-binding behaviors were investigated by absorption titration and luminescence spectra. The DNA-binding constants were 3.1 (+/- 0.2)x10(4) and 3.0 (+/- 0.2)x10(5)M(-1) for 1 and 2. The antioxidant activity of these complexes against hydroxyl radical () was explored. The cytotoxicities in vitro towards A549, BEL-7402, MG-63, and SKBR-3 cells were studied by 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Complex 1 shows high cytotoxicity, but 2 is not cytotoxic towards the selected cell lines. Apoptosis and cell cycle distribution were studied by flow cytometry. The cellular uptake showed that complexes can enter into the cytoplasm. JC-1 was used as a fluorescence probe in detecting the mitochondrial membrane potential and results indicate that the mitochondrial membrane potential decreases.

引用

页码：2423 / 2433

页数：11

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