Ectopic expression of a microbial-type rhodopsin restores visual responses in mice with photoreceptor degeneration

被引:591
作者
Bi, AD
Cui, JJ
Ma, YP
Olshevskaya, E
Pu, ML
Dizhoor, AM
Pan, ZH
机构
[1] Wayne State Univ, Sch Med, Dept Anat & Cell Biol, Detroit, MI 48201 USA
[2] Penn Coll Optometry, Elkins Pk, PA 19027 USA
[3] Peking Univ, Sch Basic Med Sci, Dept Anat & Embryol, Beijing 100083, Peoples R China
关键词
D O I
10.1016/j.neuron.2006.02.026
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The death of photoreceptor cells caused by retinal degenerative diseases often results in a complete loss of retinal responses to light. We explore the feasibility of converting inner retinal neurons to photosensitive cells as a possible strategy for imparting light sensitivity to retinas lacking rods and cones. Using delivery by an adeno-associated viral vector, here, we show that long-term expression of a microbial-type rhodopsin, channelrhodopsin-2 (ChR2), can be achieved in rodent inner retinal neurons in vivo. Furthermore, we demonstrate that expression of ChR2 in surviving inner retinal neurons of a mouse with photoreceptor degeneration can restore the ability of the retina to encode light signals and transmit the light signals to the visual cortex. Thus, expression of microbial-type channelrhodopsins, such as ChR2, in surviving inner retinal neurons is a potential strategy for the restoration of vision after rod and cone degeneration.
引用
收藏
页码:23 / 33
页数:11
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