C-scan photoacoustic microscopy for in vivo imaging of Drosophila pupae

被引：15

作者：

Xi, Lei ^{[1
]}

Zhou, Lei ^{[2
]}

Jiang, Huabei ^{[1
]}

机构：

[1] Univ Florida, Dept Biomed Engn, Gainesville, FL 32611 USA

[2] Univ Florida, Dept Mol Genet & Microbiol, Gainesville, FL 32611 USA

来源：

APPLIED PHYSICS LETTERS | 2012年 / 101卷 / 01期

关键词：

bioacoustics; biological organs; biomedical optical imaging; cellular biophysics; neurophysiology; phantoms; photoacoustic effect; zoology;

D O I：

10.1063/1.4732797

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

In this study, C-scan based high resolution photoacoustic microscopy (CPAM) is devised and used to in vivo image the DsRed-expressing cells in the central nervous system (CNS) of Drosophila pupae. The quality of out-of-focus images was improved significantly from both the phantom and in vivo experiments. Organs containing DsRed-expressing cells in Drosophila pupae can be imaged using our CPAM and agreed well with the histological sections. This technology allows rapid imaging and monitoring of organismal development in mesoscopic-scale animals as well as the generation of a detailed atlas of CNS development in the pupal stage. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4732797]

引用

页数：4

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