Cytometric analysis of perforin expression in NK cells, CD8+, and CD4+lymphocytes in children with autoimmune Hashimoto's thyroiditis - a preliminary study

被引：4

作者：

Popko, Katarzyna ^{[1
]}

Osinska, Iwona ^{[2
]}

Kucharska, Anna ^{[3
]}

Demkow, Urszula ^{[1
]}

机构：

[1] Med Univ Warsaw, Dept Lab Diagnost & Clin Immunol Dev Age, PL-00576 Warsaw, Poland

[2] Med Univ Warsaw, Fac Med 1, Dept Pathol, PL-00576 Warsaw, Poland

[3] Med Univ Warsaw, Dept Pediat & Endocrinol, PL-00576 Warsaw, Poland

来源：

JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM | 2015年 / 28卷 / 7-8期

关键词：

cytotoxicity; Hashimoto's thyroiditis; NK cells; perforin; T-CELLS; DISEASE;

D O I：

10.1515/jpem-2014-0520

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Perforin plays an essential role in cytotoxicity of natural killers (NK) and CD8+ lymphocytes. Cytotoxicity of T and NK cells is one of the mechanisms of destruction of cells in Hashimoto's disease (HD). The aim of this study was analysis of the expression of perforin in CD8+, CD4+, and NK cells and cytotoxic abilities of these cells in children with HD compared to healthy controls. The expression of perforin and surface antigens, as well as cytotoxicity were analyzed with a flow cytometry. Lower expression of perforin in CD8+ and NK was found in HD compared to controls (p = 0.01; p = 0.004). A significant correlation between perforin expression in CD8+ lymphocytes and in NK was observed (p = 0.05). The spontaneous cytotoxicity of NK was significantly higher in HD compared to controls (p = 0.04). Our results suggest that perforin plays an important role in the pathogenesis of autoimmune Hashimoto's thyroiditis.

引用

页码：789 / 792

页数：4

共 6 条

[1] Increase in activated CD8+T lymphocytes expressing perforin and granzyme B correlates with disease activity in patients with systemic lupus erythematosus [J].