Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes

被引：70

作者：

Shin, Seung Chul ^{[1
]}

Ahn, Do Hwan ^{[1
,2
]}

Kim, Su Jin ^{[3
]}

Lee, Hyoungseok ^{[1
]}

Oh, Tae-Jin ^{[4
]}

Lee, Jong Eun ^{[5
]}

Park, Hyun ^{[1
,2
]}

机构：

[1] Korea Polar Res Inst, Inchon, South Korea

[2] Univ Sci & Technol, Taejon, South Korea

[3] Korea Univ, Coll Life Sci & Biotechnol, Seoul, South Korea

[4] SunMoon Univ, Dept Pharmaceut Engn, Asan, South Korea

[5] DNALink Inc, Seoul, South Korea

来源：

PLOS ONE | 2013年 / 8卷 / 07期

关键词：

PYROSEQUENCING READS; DNA;

D O I：

10.1371/journal.pone.0068824

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.

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页数：9

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