A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation

被引：168
作者：
Muruato, Antonio E. [1 ,2 ]
Fontes-Garfias, Camila R. [1 ]
Ren, Ping [3 ]
Garcia-Blanco, Mariano A. [1 ,4 ,5 ]
Menachery, Vineet D. [2 ,3 ,6 ]
Xie, Xuping [1 ]
Shi, Pei-Yong [1 ,6 ,7 ,8 ,9 ]
机构：
[1] Univ Texas Med Branch, Dept Biochem & Mol Biol, Galveston, TX 77555 USA
[2] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA
[3] Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA
[4] Duke NUS Med Sch, Programme Emerging Infect Dis, Singapore, Singapore
[5] Univ Texas Med Branch, Dept Internal Med, Galveston, TX 77555 USA
[6] Univ Texas Med Branch, Inst Human Infect & Immun, Galveston, TX 77555 USA
[7] Univ Texas Med Branch, Sealy Inst Vaccine Sci, Galveston, TX 77555 USA
[8] Univ Texas Med Branch, Sealy Ctr Struct Biol & Mol Biophys, Galveston, TX 77555 USA
[9] Univ Texas Med Branch, Inst Translat Sci, Galveston, TX 77555 USA
来源：
NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期
关键词：
ZIKA;
D O I：
10.1038/s41467-020-17892-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Virus neutralization remains the gold standard for determining antibody efficacy. Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine development. Here, we report on a fluorescence-based SARS-CoV-2 neutralization assay that detects SARS-CoV-2 neutralizing antibodies in COVID-19 patient specimens and yields comparable results to plaque reduction neutralizing assay, the gold standard of serological testing. The fluorescence-based neutralization assay is specific to measure COVID-19 neutralizing antibodies without cross reacting with patient specimens with other viral, bacterial, or parasitic infections. Collectively, our approach offers a rapid platform that can be scaled to screen people for antibody protection from COVID-19, a key parameter necessary to safely reopen local communities.
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页数：6
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