A versatile platform for highly sensitive detection of kinase activity based on metal ion-mediated FRET using an anionic conjugated polymer

被引：36
|
作者：
Bai, Jie [1 ]
Liu, Chenghui [1 ]
Yang, Teng [1 ]
Wang, Fangfang [1 ]
Li, Zhengping [1 ]
机构：
[1] Hebei Univ, Coll Chem & Environm Sci, Key Lab Med Chem & Mol Diag, Minist Educ, Baoding 071002, Hebei Province, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 37期
基金：
中国国家自然科学基金;
关键词：
FLUORESCENCE POLARIZATION ASSAY; GOLD NANOPARTICLES; ACTIVATION; REPORTERS; SUBSTRATE; STRATEGY; PROTEASE; CAPTURE; DNA;
D O I：
10.1039/c3cc41417e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An anionic fluorescent conjugated polymer (PFPaa), coupled with the metal ion-mediated fluorescence resonance energy transfer, has been used to design a versatile platform for homogeneous detection of protein kinase activity.
引用
收藏
页码：3887 / 3889
页数：3
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