A versatile platform for highly sensitive detection of kinase activity based on metal ion-mediated FRET using an anionic conjugated polymer

被引：37

作者：

Bai, Jie ^{[1
]}

Liu, Chenghui ^{[1
]}

Yang, Teng ^{[1
]}

Wang, Fangfang ^{[1
]}

Li, Zhengping ^{[1
]}

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Key Lab Med Chem & Mol Diag, Minist Educ, Baoding 071002, Hebei Province, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 37期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENCE POLARIZATION ASSAY; GOLD NANOPARTICLES; ACTIVATION; REPORTERS; SUBSTRATE; STRATEGY; PROTEASE; CAPTURE; DNA;

D O I：

10.1039/c3cc41417e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An anionic fluorescent conjugated polymer (PFPaa), coupled with the metal ion-mediated fluorescence resonance energy transfer, has been used to design a versatile platform for homogeneous detection of protein kinase activity.

引用

页码：3887 / 3889

页数：3

共 41 条

[1] Role of tyrosine kinase inhibitors in cancer therapy [J].

Arora, A ;

Scholar, EM .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2005, 315 (03) :971-979

[2] Oncogenic kinase signalling [J].

Blume-Jensen, P ;

Hunter, T .

NATURE, 2001, 411 (6835) :355-365

[3] Design and synthesis of a fluorescent reporter of protein kinase activity [J].

Chen, CA ;

Yeh, RH ;

Lawrence, DS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (15) :3840-3841

[4] Assemblies of Conjugated Polyelectrolytes with Proteins for Controlled Protein Photoinactivation [J].

Duan, Xinrui ;

Liu, Libing ;

Feng, Xuli ;

Wang, Shu .

ADVANCED MATERIALS, 2010, 22 (14) :1602-+

[5] Cationic Conjugated Polymers for Optical Detection of DNA Methylation, Lesions, and Single Nucleotide Polymorphisms [J].

Duan, Xinrui ;

Liu, Libing ;

Feng, Fude ;

Wang, Shu .

ACCOUNTS OF CHEMICAL RESEARCH, 2010, 43 (02) :260-270

[6] Water-soluble fluorescent conjugated polymers and their interactions with biomacromolecules for sensitive biosensors [J].

Feng, Xuli ;

Liu, Libing ;

Wang, Shu ;

Zhu, Daoben .

CHEMICAL SOCIETY REVIEWS, 2010, 39 (07) :2411-2419

[7] Fluorescence Logic-Signal-Based Multiplex Detection of Nucleases with the Assembly of a Cationic Conjugated Polymer and Branched DNA [J].

Feng, Xuli ;

Duan, Xinrui ;

Liu, Libing ;

Feng, Fude ;

Wang, Shu ;

Li, Yuliang ;

Zhu, Daoben .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (29) :5316-5321

[8] Probing Protein Kinase (CK2) and Alkaline Phosphatase with CdSe/ZnS Quantum Dots [J].

Freeman, Ronit ;

Finder, Tali ;

Gill, Ron ;

Willner, Itamar .

NANO LETTERS, 2010, 10 (06) :2192-2196

[9] A homogeneous fluorescence polarization assay adaptable for a range of protein serine/threonine and tyrosine kinases [J].

Gaudet, EA ;

Huang, KS ;

Zhang, Y ;

Huang, W ;

Mark, D ;

Sportsman, JR .

JOURNAL OF BIOMOLECULAR SCREENING, 2003, 8 (02) :164-175

[10] Kinase-Actuated Immunoaggregation of Peptide-Conjugated Gold Nanoparticles [J].

Gupta, Shalini ;

Andresen, Heiko ;

Ghadiali, James E. ;

Stevens, Molly M. .

SMALL, 2010, 6 (14) :1509-1513

← 1 2 3 4 5 →