Accumulation of nuclear-encoded tRNA(Thr)(AGU) in mitochondria of the liverwort Marchantia polymorpha

被引：12
作者：
Akashi, K [1 ]
Hirayama, J [1 ]
Takenaka, M [1 ]
Yamaoka, S [1 ]
Suyama, Y [1 ]
Fukuzawa, H [1 ]
Ohyama, K [1 ]
机构：
[1] KYOTO UNIV,FAC AGR,DEPT AGR CHEM,PLANT MOL BIOL LAB,SAKYO KU,KYOTO 60601,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1350卷 / 03期
基金：
日本学术振兴会;
关键词：
mitochondrial tRNA; nuclear tRNA(Thr) gene; tRNA import; (Marchantia polymorpha);
D O I：
10.1016/S0167-4781(96)00239-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mitochondrial genome of the liverwort Marchantia polymorpha does not encode the full complement of tRNAs for the threonine and isoleucine codon boxes. To find the missing tRNA genes specifically for tRNA(Thr) in mitochondria, we have searched the genomic library and identified two clones (pTT1 and pTT2), encoding the identical tRNA(Thr)(AGU) gene copy with different 5'- and 3'-flanking sequences. By northern analysis, we demonstrate considerable accumulation of the nuclear encoded tRNA(Thr) and moderate expression of native tRNA(Thr)(GGU) in mitochondria. Nonetheless, the imported and native tRNA(Thr) species together are not sufficient to translate all four threonine codons used in liverwort mitochondria, implicating mitochondrial import of at least one additional threonine isoacceptor tRNA. (C) 1997 Elsevier Science B.V.
引用
收藏
页码：262 / 266
页数：5
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