Streptavidin-enhanced surface plasmon resonance biosensor for highly sensitive and specific detection of microRNA

被引：57

作者：

Zhang, Decai ^{[1
]}

Yan, Yurong ^{[1
]}

Cheng, Wei ^{[2
]}

Zhang, Wei ^{[1
]}

Li, Yahui ^{[1
]}

Ju, Huangxian ^{[1
,3
]}

Ding, Shijia ^{[1
]}

机构：

[1] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 1, Mol Oncol & Epigenet Lab, Chongqing 400016, Peoples R China

[3] Nanjing Univ, Dept Chem, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China

来源：

MICROCHIMICA ACTA | 2013年 / 180卷 / 5-6期

基金：

中国国家自然科学基金;

关键词：

Surface plasmon resonance; Biosensor; MicroRNA; Singal amplification; Streptavidin; LABEL-FREE DETECTION; RAMAN-SPECTROSCOPY; EXPRESSION; DNA; PROBES; QUANTIFICATION; HYBRIDIZATION; GRAPHENE; TARGETS; SENSORS;

D O I：

10.1007/s00604-013-0945-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We are presenting a method for sensitive and specific detection of microRNA (miRNA) using surface plasmon resonance. A thiolated capture DNA probe with a short complete complementary sequence was immobilized on the gold surface of the sensor to recognize the part sequence of target miRNA, and then an oligonucleotide probe linked to streptavidin was employed to bind the another section of the target. The use of the streptavidin-oligonucleotide complex caused a similar to 5-fold increase in signal, improved the detection sensitivity by a factor of similar to 24, and lowered the detection limit to 1.7 fmol of miR-122. This specificity allowed a single mismatch in the target miRNA to be discriminated. The whole assay takes 30 min, and the surface of the sensor can be regenerated at least 30 times without loss in performance. The method was successfully applied to the determination of miRNA spiked into human total RNA samples.

引用

页码：397 / 403

页数：7

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