Reduced graphene oxide-based field effect transistors for the detection of E7 protein of human papillomavirus in saliva

被引：88

作者：

Aspermair, Patrik ^{[1
,2
,3
]}

Mishyn, Vladyslav ^{[1
]}

Bintinger, Johannes ^{[3
]}

Happy, Henri ^{[1
]}

Bagga, Komal ^{[4
]}

Subramanian, Palaniappan ^{[5
]}

Knoll, Wolfgang ^{[2
,3
]}

Boukherroub, Rabah ^{[1
]}

Szunerits, Sabine ^{[1
]}

机构：

[1] Univ Lille, CNRS, Cent Lille, Univ Polytech Hauts de France,UMR 8520,IEMN, F-59000 Lille, France

[2] CEST Competence Ctr Electrochem Surface Technol, Konrad Lorenz Str 24, A-3430 Tulln, Austria

[3] Austrian Inst Technol, Biosensor Technol, Konrad Lorenz Str 24, A-3430 Tulln, Austria

[4] Dublin City Univ, Sch Mech & Mfg Engn, Adv Proc Technol Res Ctr, Dublin 9, Ireland

[5] Katholieke Univ Leuven, Dept Mat Engn, Kateelpk, B-3001 Heverlee, Belgium

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2021年 / 413卷 / 03期

关键词：

Human papillomavirus (HPV); Aptamer; Reduced graphene oxide (rGO); Field effect transistors; Surface functionalization; Electronic biosensor; Point-of-care diagnostics; VIRAL DETECTION; BIOSENSOR; MOBILITY; APTAMER; PCR;

D O I：

10.1007/s00216-020-02879-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Several challenging biological sensing concepts have been realized using electrolyte-gated reduced graphene oxide field effect transistors (rGO-FETs). In this work, we demonstrate the interest of rGO-FET for the sensing of human papillomavirus (HPV), one of the most common sexually transmitted viruses and a necessary factor for cervical carcinogenesis. The highly sensitive and selective detection of the HPV-16 E7 protein relies on the attractive semiconducting characteristics of pyrene-modified rGO functionalized with RNA aptamer Sc5-c3. The aptamer-functionalized rGO-FET allows for monitoring the aptamer-HPV-16 E7 protein binding in real time with a detection limit of about 100 pg mL(-1)(1.75 nM) for HPV-16 E7 from five blank noise signals (95% confidence level). The feasibility of this method for clinical application in point-of-care technology is evaluated using HPV-16 E7 protein suspended in saliva and demonstrates the successful fabrication of a promising field effect transistor biosensor for HPV diagnosis. Graphical abstract

引用

页码：779 / 787

页数：9

共 36 条

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