In Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with Fluorescent D-Amino Acids

被引：493

作者：

Kuru, Erkin ^{[1
]}

Hughes, H. Velocity ^{[1
]}

Brown, Pamela J. ^{[1
]}

Hall, Edward ^{[1
]}

Tekkam, Srinivas ^{[1
]}

Cava, Felipe ^{[2
]}

de Pedro, Miguel A. ^{[2
]}

Brun, Yves V. ^{[1
]}

VanNieuwenhze, Michael S. ^{[1
]}

机构：

[1] Indiana Univ, Bloomington, IN 47405 USA

[2] Univ Autonoma Madrid, E-28049 Madrid, Spain

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 50期

基金：

美国国家卫生研究院;

关键词：

bacteria; biosensors; D-amino acids; fluorescent probes; peptidoglycan; GRAM-NEGATIVE BACTERIA; CELL-WALL; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; DISTINCT; GROWTH; SHAPE;

D O I：

10.1002/anie.201206749

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC=7-hydroxycoumarin, NBD=7-nitrobenzofurazan, TAMRA= carboxytetramethylrhodamine. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

收藏

页码：12519 / 12523

页数：5

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