Structural characterisation and antimutagenic activity of a novel polysaccharide isolated from Sepiella maindroni ink

被引：97

作者：

Liu, Chunhui ^{[1
,2
]}

Li, Xiaodong ^{[1
]}

Li, Yuhua ^{[1
]}

Feng, You ^{[1
]}

Zhou, Shuai ^{[1
]}

Wang, Fengshan ^{[1
,2
]}

机构：

[1] Shandong Univ, Sch Pharmaceut Sci, Inst Biochem & Biotechnol Drugs, Jinan 250012, Peoples R China

[2] Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250012, Peoples R China

来源：

FOOD CHEMISTRY | 2008年 / 110卷 / 04期

基金：

中国博士后科学基金;

关键词：

cuttlefish; Sepiella maindroni; ink; polysaccharide; structure; antimutagenic activity;

D O I：

10.1016/j.foodchem.2008.02.026

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A new heteropolysaccharide, named as SIP, was isolated from the ink of cuttlefish, Sepiella maindroni, by enzymolysis, anion-exchange and gel-permeation chromatography and tested for its antimutagenic activity. It was homogeneous with a molecular weight of 1.13 x 10(4) Da by HPSEC-MALLS analysis. SIP contained glucuronic acid, mannose, N-acetylgalactosamine, and fucose in a molar ratio of 1:1:2:2. Its structural characteristics were investigated and elucidated by methylation analysis, GLC-MS, and NMR (H-1, C-13, H-H COSY, HMQC, HMBC, TOCSY and NOESY). The hexasaccharide repeating unit of SIP was found to be a backbone composed of fucose, N-acetylgalactosamine and mannose in a molar ratio of 2:2:1, and with a single branch of glucuronic acid at the C-3 position of mannose. According to the micronucleus test, SIP could significantly reduce the frequency of micronucleated cells in polychromatic erythrocytes and reticulocytes induced by cyclophosphamide in tumor-bearing mice, which revealed that SIP presented strong antimutagenic activity. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：807 / 813

页数：7

共 20 条

[1] CYCLOPHOSPHAMIDE - REVIEW OF ITS MUTAGENICITY FOR AN ASSESSMENT OF POTENTIAL GERM-CELL RISKS [J].