Mild acid pretreatment and enzyme saccharification of Sorghum bicolor straw

被引：47

作者：

Vancov, T. ^{[1
,2
]}

McIntosh, S. ^{[1
]}

机构：

[1] Wollongbar Primary Ind Inst, Ind & Investment NSW, Wollongbar, NSW 2477, Australia

[2] Univ New England, Primary Ind Innovat Ctr, Armidale, NSW 2351, Australia

来源：

APPLIED ENERGY | 2012年 / 92卷

关键词：

Acid pretreatment; Cellulases; Enzyme saccharification; Inhibitors; Lignocellulose; Sorghum straw; CORN STOVER; ETHANOL-PRODUCTION; FERMENTATION; INHIBITION; HYDROLYSIS; OPTIMIZATION; BIOETHANOL; CELLULOSE; SEVERITY; SOFTWOOD;

D O I：

10.1016/j.apenergy.2011.11.053

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

Dilute sulphuric acid pretreatment followed by enzyme saccharification of Sorghum bicolor straw was undertaken to examine its potential as a feedstock in bioethanol production in Australia. Factorial design experiments evaluated the impact of pretreatment parameters on hemicellulose solubilisation and cellulose enzymatic hydrolysis. Sugar yields in prehydrolysate and saccharified liquors were found to increase with treatment severity; temperature was found to have the greatest impact. Degradation products were minimal; acetate and total phenolics peaked at 33 and 1.5 mg/g respectively. Conditions for maximum hemicellulose solubilisation (2% H2SO4 for 60 min at 121 degrees C) differed to those associated with maximum glucose release from solid residue saccharifications (1% H2SO4/90 min /121 degrees C). Water extractive sugars accounted for over 20% total sugars recovered. Addition of beta-glucosidase and xylanase to enzyme saccharification enhanced reaction rates and final sugar yields three-fold, whilst reducing cellulase dosage. Considering its abundance, high sugar potential and apparent ease of conversion, sorghum straw is an appropriate feedstock for the production of second generation fuels. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

引用

页码：421 / 428

页数：8

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