Insignificant Difference in Biocompatibility of Regenerated Silk Fibroin Prepared with Ternary Reagent Compared with Regenerated Silk Fibroin Prepared with Lithium Bromide

被引:12
作者
Cheng, Guotao [1 ]
Wang, Xin [2 ]
Wu, Mengqiu [1 ]
Wu, Siyuan [1 ]
Cheng, Lan [1 ]
Zhang, Xiaoning [1 ]
Dai, Fangyin [1 ]
机构
[1] Southwest Univ, Coll Sericulture & Text & Biomass Sci, State Key Lab Silkworm Genome Biol, Minist Agr & Rural Affairs,Key Lab Sericultural B, Chongqing 400715, Peoples R China
[2] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Ctr Joint Surg, Chongqing 400038, Peoples R China
关键词
silk fibroin; dissolution; ternary reagent; lithium bromide; biocompatibility; hemolysis; MOLECULAR-WEIGHT; AMINO-ACIDS; DISSOLUTION; CONFORMATION; PERFORMANCE; FIBERS; IONS; CA2+; PH;
D O I
10.3390/polym14183903
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Bombyx mori silk fibroin (SF) is widely used in the field of biomaterials due to its excellent biocompatibility and mechanical properties. However, SF cannot be used directly in many applications and needs to be dissolved first. Lithium bromide (LiBr) is a traditional solvent which is usually used to dissolve SF. However, LiBr has several limitations, e.g., it is expensive, it is toxic to organisms, and it is environmentally unfriendly. Herein, we investigate the possibility of developing a ternary reagent system that is inexpensive, non-toxic to organisms, and environmentally friendly as an alternative for silk fibroin solubilization. The results confirm that regenerated silk fibroin (RSF) prepared using a ternary reagent has the same morphology and amino acid composition as that prepared using LiBr, but the RSF prepared using a ternary reagent still had a small amount of calcium residue even after long-term dialysis. Further research found that the residual calcium does not cause significant differences in the structure and biological performance of the RSF, such as its cytotoxicity, blood compatibility, and antibacterial properties. Therefore, we believe that ternary reagents are an ideal alternative solvent for dissolving SF.
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页数:13
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