Helicobacter pylori infection assessed by ELISA and by immunoblot and noncardia gastric cancer risk in a prospective study: the Eurgast-EPIC project

被引：103

作者：

Gonzalez, C. A. ^{[1
]}

Megraud, F. ^{[2
]}

Buissonniere, A. ^{[2
]}

Lujan Barroso, L. ^{[1
]}

Agudo, A. ^{[1
]}

Duell, E. J. ^{[1
]}

Boutron-Ruault, M. C. ^{[3
,4
]}

Clavel-Chapelon, F. ^{[3
,4
]}

Palli, D. ^{[5
]}

Krogh, V. ^{[6
]}

Mattiello, A. ^{[7
]}

Tumino, R. ^{[8
,9
]}

Sacerdote, C. ^{[10
]}

Quiros, J. R. ^{[11
]}

Sanchez-Cantalejo, E. ^{[12
]}

Navarro, C. ^{[13
]}

Barricarte, A. ^{[14
]}

Dorronsoro, M. ^{[15
]}

Khaw, K. -T. ^{[16
]}

Wareham, N. ^{[16
]}

Allen, N. E. ^{[17
]}

Tsilidis, K. K. ^{[17
]}

Bueno-de-Mesquita, H. Bas ^{[18
,19
]}

Jeurnink, S. M. ^{[18
,19
]}

Numans, M. E. ^{[20
]}

Peeters, P. H. M. ^{[20
]}

Lagiou, P. ^{[21
]}

Valanou, E. ^{[22
]}

Trichopoulou, A. ^{[22
]}

Kaaks, R. ^{[23
]}

Lukanova-McGregor, A. ^{[23
]}

Bergman, M. M. ^{[24
]}

Boeing, H. ^{[24
]}

Manjer, J. ^{[25
]}

Lindkvist, B. ^{[26
]}

Stenling, R. ^{[27
]}

Hallmans, G. ^{[28
]}

Mortensen, L. M. ^{[29
]}

Overvad, K. ^{[29
]}

Olsen, A. ^{[30
]}

Tjonneland, A. ^{[30
]}

Bakken, K. ^{[31
]}

Dumeaux, V. ^{[31
]}

Lund, E. ^{[31
]}

Jenab, M. ^{[32
]}

Romieu, I. ^{[32
]}

Michaud, D. ^{[33
]}

Mouw, T. ^{[33
]}

Carneiro, F. ^{[34
,35
]}

Fenge, C. ^{[36
]}

机构：

[1] Catalan Inst Oncol, Bellvitge Biomed Res Inst IDIBELL, Canc Epidemiol Res Program, Unit Nutr Environm & Canc, Barcelona, Spain

[2] INSERM, U853, Bordeaux, France

[3] Inst Gustave Roussy, Ctr Res Epidemiol & Populat Hlth, Villejuif, France

[4] Paris S Univ, Villejuif, France

[5] Canc Res & Prevent Inst ISPO, Mol & Nutr Epidemiol Unit, Florence, Italy

[6] Fdn IRCCS Ist Nazl Tumori, Dept Prevent & Predict Med, Nutr Epidemiol Unit, Milan, Italy

[7] Univ Naples Federico II, Dept Clin & Expt Med, Naples, Italy

[8] Civile MP Arezzo Hosp, Canc Registry, Ragusa, Spain

[9] Civile MP Arezzo Hosp, Histopathol Unit, Ragusa, Spain

[10] Ctr Canc Epidemiol & Prevent CPO Piemonte, Turin, Italy

[11] Hlth & Hlth Care Serv Council, Publ Hlth & Participat Directorate, Asturias, Spain

[12] CIBER Epidemiol & Salud Publ CIBERESP, Andalusian Sch Publ Hlth, Granada, Spain

[13] CIBER Epidemiol & Salud Publ CIBERESP Murcia, Murcia Hlth Council, Dept Epidemiol, Murcia, Spain

[14] CIBER Epidemiol & Salud Publ CIBERESP, Navarre Publ Hlth Inst, Pamplona, Spain

[15] Basque Reg Hlth Dept, Publ Hlth Div Gipuzkoa & Ciberesp, San Sebastian, Spain

[16] Univ Cambridge, Dept Publ Hlth & Primary Care, Cambridge, England

[17] Univ Oxford, Nuffield Dept Clin Med, Canc Epidemiol Unit, Oxford, England

[18] Natl Inst Publ Hlth & Environm RIVM, Bilthoven, Netherlands

[19] Univ Med Ctr Utrecht UMCU, Dept Gastroenterol & Hepatol, Utrecht, Netherlands

[20] Univ Med Ctr Utrecht, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands

[21] Univ Athens, Sch Med, Dept Hyg Epidemiol & Med Stat, WHO Collaborating Ctr Food & Nutr Policies, GR-11527 Athens, Greece

[22] Hellen Hlth Fdn, Athens, Greece

[23] German Canc Res Ctr, Dept Canc Epidemiol, D-6900 Heidelberg, Germany

[24] German Inst Human Nutr Potsdam Rehbrucke, Dept Epidemiol, Potsdam, Germany

[25] Lund Univ, Skane Univ Hosp Malmo, Dept Surg, Malmo, Sweden

[26] Sahlgrens Univ Hosp, Dept Internal Med, Div Gastroenterol Hepatol, Gothenburg, Sweden

[27] Umea Univ, Dept Med Biosci, Umea, Sweden

[28] Umea Univ, Dept Publ Hlth & Clin Med, Umea, Sweden

[29] Aarhus Univ, Sch Publ Hlth, Dept Epidemiol, Aarhus, Denmark

[30] Danish Canc Soc, Inst Canc Epidemiol Diet Canc & Hlth, Copenhagen, Denmark

[31] Univ Tromso, Dept Community Med, Tromso, Norway

[32] Int Agcy Res Canc IARC WHO, Lyon, France

[33] Univ London Imperial Coll Sci Technol & Med, Sch Publ Hlth, Dept Epidemiol & Biostat, London, England

[34] Univ Porto IPATIMUP, Inst Mol Pathol & Immunol, Oporto, Portugal

[35] Med Fac HS Joao, Oporto, Portugal

[36] Odense Univ Hosp, Dept Clin Pathol, DK-5000 Odense, Denmark

来源：

ANNALS OF ONCOLOGY | 2012年 / 23卷 / 05期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

Helicobacter pylori; noncardia gastric cancer; prospective study; western blot; CAGA;

D O I：

10.1093/annonc/mdr384

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In epidemiological studies, Helicobacter pylori infection is usually detected by enzyme-linked immunosorbent assay (ELISA). However, infection can spontaneously clear from the mucosa during the progression of atrophy and could lead to substantial under-detection of infection and underestimation of its effect on gastric cancer (GC) risk. Antibodies detected by western blot are known to persist longer after the loss of the infection. In a nested case-control study from the Eurogast-EPIC cohort, including 88 noncardia GC cases and 338 controls, we assessed the association between noncardia GC and H. pylori infection comparing antibodies detected by western blot (HELICOBLOT2.1) to those detected by ELISA (Pyloriset EIA-GIII((R))). By immunoblot, 82 cases (93.2%) were H. pylori positive, 10 of these cases (11.4%) were negative by ELISA and only 6 cases (6.8%) were negative by both ELISA and immunoblot. Multivariable odds ratio (OR) for noncardia GC comparing immunoglobulin G positive versus negative by ELISA was 6.8 [95% confidence interval (CI) 3.0-15.1], and by immunoblot, the OR was 21.4 (95% CI 7.1-64.4). Using a western blot assay, nearly all noncardia GC were classified as H. pylori positive and the OR was more than threefold higher than the OR assessed by ELISA, supporting the hypothesis that H. pylori infection is a necessary condition for noncardia GC.

引用

页码：1320 / 1324

页数：5

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