MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice

被引：188

作者：

Horie, Takahiro ^{[1
,2
]}

Nishino, Tomohiro ^{[1
]}

Baba, Osamu ^{[1
]}

Kuwabara, Yasuhide ^{[1
]}

Nakao, Tetsushi ^{[1
]}

Nishiga, Masataka ^{[1
]}

Usami, Shunsuke ^{[1
]}

Izuhara, Masayasu ^{[1
]}

Sowa, Naoya ^{[1
]}

Yahagi, Naoya ^{[3
,4
]}

Shimano, Hitoshi ^{[3
,4
]}

Matsumura, Shigenobu ^{[5
]}

Inoue, Kazuo ^{[5
]}

Marusawa, Hiroyuki ^{[6
]}

Nakamura, Tomoyuki ^{[7
]}

Hasegawa, Koji ^{[8
]}

Kume, Noriaki ^{[9
]}

Yokode, Masayuki ^{[2
]}

Kita, Toru ^{[10
]}

Kimura, Takeshi ^{[1
]}

Ono, Koh ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Kyoto 6068507, Japan

[2] Kyoto Univ, Grad Sch Med, Inst Adv Clin & Translat Sci, Dept Clin Innovat Med, Kyoto 6068507, Japan

[3] Univ Tsukuba, Grad Sch Comprehens Human Sci, Dept Internal Med Endocrinol & Metab, Nutrigen Res Grp,Fac Med, Tsukuba, Ibaraki 3058575, Japan

[4] Univ Tsukuba, Int Inst Integrat Sleep Med IIIS, World Premir Int Res Ctr Initiat WPI, Tsukuba, Ibaraki 3058575, Japan

[5] Kyoto Univ, Grad Sch Agr, Div Food Sci & Biotechnol, Nutr Chem Lab, Kyoto 6068502, Japan

[6] Kyoto Univ, Grad Sch Med, Dept Gastroenterol, Kyoto 6068507, Japan

[7] Kansai Med Univ, Dept Pharmacol, Moriguchi, Osaka 5708506, Japan

[8] Natl Hosp Org, Kyoto Med Ctr, Div Translat Res, Kyoto 6128555, Japan

[9] Kobe Gakuin Univ, Fac Pharmaceut Sci, Div Clin Pharm, Kobe, Hyogo 6508586, Japan

[10] Kobe City Med Ctr Gen Hosp, Dept Cardiovasc Med, Kobe, Hyogo 6500046, Japan

来源：

NATURE COMMUNICATIONS | 2013年 / 4卷

基金：

日本学术振兴会;

关键词：

INDUCED HEPATIC STEATOSIS; FATTY-ACID SYNTHESIS; PPAR-GAMMA; CHOLESTEROL HOMEOSTASIS; TRANSCRIPTION FACTOR; INSULIN-RESISTANCE; CULTURED-CELLS; RECEPTOR; MIR-33; LIVER;

D O I：

10.1038/ncomms3883

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNAs (miRs) are small non-protein-coding RNAs that bind to specific mRNAs and inhibit translation or promote mRNA degradation. Recent reports have indicated that miR-33, which is located within the intron of sterol regulatory element-binding protein (SREBP) 2, controls cholesterol homoeostasis and may be a potential therapeutic target for the treatment of atherosclerosis. Here we show that deletion of miR-33 results in marked worsening of high-fat diet-induced obesity and liver steatosis. Using miR-33(-/-) Srebf1(+/-) mice, we demonstrate that SREBP-1 is a target of miR-33 and that the mechanisms leading to obesity and liver steatosis in miR-33(-/-) mice involve enhanced expression of SREBP-1. These results elucidate a novel interaction between SREBP-1 and SREBP-2 mediated by miR-33 in vivo.

引用

页数：12

共 44 条

[1] miR-33 controls the expression of biliary transporters, and mediates statin- and diet-induced hepatotoxicity [J].

Allen, Ryan M. ;

Marquart, Tyler J. ;

Albert, Carolyn J. ;

Suchy, Frederick J. ;

Wang, David Q. -H. ;

Ananthanarayanan, Meenakshisundaram ;

Ford, David A. ;

Baldan, Angel .

EMBO MOLECULAR MEDICINE, 2012, 4 (09) :882-895

[2] The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor [J].

Brown, MS ;

Goldstein, JL .

CELL, 1997, 89 (03) :331-340

[3] Expression of sterol regulatory element-binding protein 1c (SREBP-1c) mRNA in rat hepatoma cells requires endogenous LXR ligands [J].

DeBose-Boyd, RA ;

Ou, JF ;

Goldstein, JL ;

Brown, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (04) :1477-1482

[4] Insulin activates human sterol-regulatory-element-binding protein-1c (SREBP-1c) promoter through SRE motifs [J].

Dif, Nicolas ;

Euthine, Vanessa ;

Gonnet, Estelle ;

Laville, Martine ;

Vidal, Hubert ;

Lefai, Etienne .

BIOCHEMICAL JOURNAL, 2006, 400 :179-188

[5] SREBP transcription factors:: master regulators of lipid homeostasis [J].

Eberlé, D ;

Hegarty, B ;

Bossard, P ;

Ferré, P ;

Foufelle, F .

BIOCHIMIE, 2004, 86 (11) :839-848

[6] Regulation of sterol synthesis in eukaryotes [J].

Espenshade, Peter J. ;

Hughes, Adam L. .

ANNUAL REVIEW OF GENETICS, 2007, 41 :401-427

[7]

Fajas L, 1999, MOL CELL BIOL, V19, P5495

[8]

FOLCH J, 1957, J BIOL CHEM, V226, P497

[9] Sterol regulatory element-binding protein-1 is regulated by glucose at the transcriptional level [J].

Hasty, AH ;

Shimano, H ;

Yahagi, N ;

Amemiya-Kudo, M ;

Perrey, S ;

Yoshikawa, T ;

Osuga, J ;

Okazaki, H ;

Tamura, Y ;

Iizuka, Y ;

Shionoiri, F ;

Ohashi, K ;

Harada, K ;

Gotoda, T ;

Nagai, R ;

Ishibashi, S ;

Yamada, N .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (40) :31069-31077

[10] Cholesterol regulation of receptor-interacting protein 140 via microRNA-33 in inflammatory cytokine production [J].

Ho, Ping-Chih ;

Chang, Kun-Che ;

Chuang, Ya-Shan ;

Wei, Li-Na .

FASEB JOURNAL, 2011, 25 (05) :1758-1766

← 1 2 3 4 5 →