The relationship of oxygen uptake rate and kLa with rheological properties in high cell density cultivation of docosahexaenoic acid by Schizochytrium sp S31

被引：41

作者：

Chang, Guifang ^{[1
,2
]}

Wu, Juan ^{[2
]}

Jiang, Cuihong ^{[2
]}

Tian, Guiwei ^{[2
]}

Wu, Qinghang ^{[2
]}

Chang, Ming ^{[1
]}

Wang, Xingguo ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Synerget Innovat Ctr Food Safety & Nutr, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[2] Wilmar Biotechnol Res Dev Ctr Ltd, Shanghai, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2014年 / 152卷

关键词：

DHA; Schizochytrium sp; k(L)a; OUR; Rheological property; MASS-TRANSFER COEFFICIENT; EXTRACELLULAR POLYSACCHARIDE; FERMENTATION PROCESSES; LIMACINUM SR21; SCALE-UP; PREDICTION; BROTH; GROWTH;

D O I：

10.1016/j.biortech.2013.11.002

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Three independent cultures by fed batch strategy under different oxygen supply levels were investigated with Schizochytrium sp. S31 on glycerol in 50 L bioreactor. Three cultures all achieved high cell density cultivation (HCDC) with more than 100 g/L biomass density. However, the culture with middle oxygen supply level achieved the highest DHA concentration at 21.26 g/L. Dissolved oxygen (DO) limitation was commonly encountered in the present cultures, which was due to the dramatic decrease of k(L)a in high oxygen supply culture resulted from significantly increasing apparent viscosity of the broth. The rheological properties of the three cultures all exhibited shear-thinning behavior. The oxygen uptake rate (OUR) predominately influenced by k(L)a was suggested to replace DO as on-line control parameter for scale-up production of DHA. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：234 / 240

页数：7

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