Validation of liquid/liquid extraction method coupled with HPLC-UV for measurement of ribavirin plasma levels in HCV-positive patients

被引：52

作者：

D'Avolio, A ^{[1
]}

Ibañez, A ^{[1
]}

Sciandra, M ^{[1
]}

Siccardi, M ^{[1
]}

de Requena, DG ^{[1
]}

Bonora, S ^{[1
]}

Di Perri, G ^{[1
]}

机构：

[1] Univ Turin, Amedeo Savoia Hosp, Dept Infect Dis, I-16410149 Turin, Italy

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 835卷 / 1-2期

关键词：

ribavirin; liquid/liquid; HPLC; UV method;

D O I：

10.1016/j.jchromb.2006.03.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Measurement of ribavirin plasma levels in HCV-positive patients have been shown to be useful in order to optimise individual ribavirin exposure. Efficacy and toxicity of this drug are shown to be concentration-dependant. A simple HPLC-UV method was developed and validated, which has an easy liquid/liquid extraction, sensitive limit of detection, without any interference peaks, reproducible and linear over the range of clinical relevant concentrations. The assay warrants further evaluation as a tool for ribavirin therapeutic drug monitoring in HCV-positive patients. (c) 2006 Published by Elsevier B.V.

引用

页码：127 / 130

页数：4

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