Vibrational spectroscopic studies of the divalent metal ion effect on DNA structural transitions

被引：23

作者：

Kornilova, SV ^{[1
]}

Miskovsky, P ^{[1
]}

Tomkova, A ^{[1
]}

Kapinos, LE ^{[1
]}

Hackl, EV ^{[1
]}

Andrushchenko, VV ^{[1
]}

Grigoriev, DN ^{[1
]}

Blagoi, YP ^{[1
]}

机构：

[1] SAFARIK UNIV,KOSICE 04154,SLOVAKIA

来源：

JOURNAL OF MOLECULAR STRUCTURE | 1997年 / 408卷

关键词：

DNA; divalent metal ions; Raman spectroscopy;

D O I：

10.1016/S0022-2860(96)09671-8

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Raman spectroscopy is used to study DNA complexes with Cu2+, Mn2+ and Ca2+ ions in 0.001 M NaCl, pH 7.0. The changes observed in the spectra permit us to conclude that all the ions interact both with the DNA phosphate groups and with the nucleic bases, changing the DNA structure. Ca2+ and Mn2+ ions bind to N7 of guanine and adenine only, but Cu2+ ions can probably bind to N7, 06 of guanine and N3 of cytosine as well. The latter type of binding occurs as the base is turning around the glycoside bonds and a G-C pair transition from anti to syn conformation takes place. Besides, at high concentrations of Ca2+ ions a partial B-A transition takes place in DNA. (C) 1997 Elsevier Science B.V.

引用

页码：219 / 223

页数：5

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