Potency under pressure: the impact of hydrostatic pressure on antigenic properties of influenza virus hemagglutinin

被引:3
作者
Eichelberger, Schafer L. [1 ]
Sultana, Ishrat [2 ]
Gao, Jin [2 ]
Getie-Kebtie, Melkamu [1 ]
Alterman, Michail [1 ]
Eichelberger, Maryna C. [2 ]
机构
[1] Off Cell Tissue & Gene Therapy, Div Cellular & Gene Therapies, Bethesda, MD USA
[2] US FDA, Div Viral Prod, Off Vaccine Res & Review, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA
关键词
Hemagglutinin; hydrostatic pressure; immunogenicity; influenza; potency; SINGLE-RADIAL-IMMUNODIFFUSION; IMMUNODEFICIENCY-VIRUS; SUBUNIT VACCINE; MEMBRANE-FUSION; INACTIVATION; ASSAY; NEURAMINIDASE; PROTECTION; INFECTION; ANTIBODY;
D O I
10.1111/irv.12102
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background Influenza vaccines are effective in protecting against illness and death caused by this seasonal pathogen. The potency of influenza vaccines is measured by single radial immunodiffusion (SRID) assay that quantifies antigenic forms of hemagglutinin (HA). Hydrostatic pressure results in loss of binding of influenza virus to red blood cells, but it is not known whether this infers loss of potency. Objectives Our goal was to determine the impact of pressure on HA antigenic structure. Methods Viruses included in the 2010-2011 trivalent influenza vaccine were subjected to increasing number of cycles at 35 000 psi in a barocycler, and the impact of this treatment measured by determining hemagglutination units (HAU) and potency. Potency was assessed by SRID and immunogenicity in mice. Results After 25 cycles of pressure, the potency measured by SRID assay was below the limit of quantification for the H1N1 and B viruses used in our study, while the H3N2 component retained some potency that was lost after 50 pressure cycles. Pressure treatment also resulted in loss of HAU, but this did not strictly correlate with the potency value. Curiously, loss of potency was abrogated when influenza A, but not B, antigens were exposed to pressure in chicken egg allantoic fluid. Protection against pressure appeared to be mediated by specific interactions because addition of bovine serum albumin did not have the same effect. Conclusions Our results show that pressure-induced loss of potency is strain dependent and suggests that pressure treatment may be useful for identifying vaccine formulations that improve HA stability.
引用
收藏
页码:961 / 968
页数:8
相关论文
共 33 条
  • [1] Influenza neuraminidase
    Air, Gillian M.
    [J]. INFLUENZA AND OTHER RESPIRATORY VIRUSES, 2012, 6 (04) : 245 - 256
  • [2] Rational design of an influenza subunit vaccine powder with sugar glass technology: Preventing conformational changes of haemagglutinin during freezing and freeze-drying
    Amorij, J.-P.
    Meulenaar, J.
    Hinrichs, W. L. J.
    Stegmann, T.
    Huckriede, A.
    Coenen, F.
    Frijlink, H. W.
    [J]. VACCINE, 2007, 25 (35) : 6447 - 6457
  • [3] A2 (N2) NEURAMINIDASE OF X-7 INFLUENZA-VIRUS RECOMBINANT - DETERMINATION OF MOLECULAR-SIZE AND SUBUNIT COMPOSITION OF ACTIVE UNIT
    BUCHER, DJ
    KILBOURNE, ED
    [J]. JOURNAL OF VIROLOGY, 1972, 10 (01) : 60 - +
  • [4] Stability of influenza sub-unit vaccine - Does a couple of days outside the refrigerator matter?
    Coenen, F
    Tolboom, JTBM
    Frijlink, HW
    [J]. VACCINE, 2006, 24 (04) : 525 - 531
  • [5] Hydration and packing effects on prion folding and β-sheet conversion -: High pressure spectroscopy and pressure perturbation calorimetry studies
    Cordeiro, Y
    Kraineva, J
    Ravindra, R
    Lima, LMTR
    Gomes, MPB
    Foguel, D
    Winter, R
    Silva, JL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (31) : 32354 - 32359
  • [6] DOMS RW, 1985, J BIOL CHEM, V260, P2973
  • [7] Dissociation of amyloid fibrils of α-synuclein and transthyretin by pressure reveals their reversible nature and the formation of water-excluded cavities
    Foguel, D
    Suarez, MC
    Ferrao-Gonzales, AD
    Porto, TCR
    Palmieri, L
    Einsiedler, CM
    Andrade, LR
    Lashuel, HA
    Lansbury, PT
    Kelly, JW
    Silva, JL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (17) : 9831 - 9836
  • [8] Hydrostatic pressure induces the fusion-active state of enveloped viruses
    Gaspar, LP
    Silva, ACB
    Gomes, AMO
    Freitas, MS
    Bom, APDA
    Schwarcz, WD
    Mestecky, J
    Novak, MJ
    Foguel, D
    Silva, JL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (10) : 8433 - 8439
  • [9] Pressure-inactivated yellow fever 17DD virus:: Implications for vaccine development
    Gaspar, Luciane P.
    Mendes, Ygara S.
    Yamamura, Anna M. Y.
    Almeida, Luiz F. C.
    Caride, Elena
    Goncalves, Rafael B.
    Silva, Jerson L.
    Oliveira, Andrea C.
    Galler, Ricardo
    Freire, Marcos S.
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2008, 150 (1-2) : 57 - 62
  • [10] High Hydrostatic Pressure Processing of Murine Norovirus 1-Contaminated Oysters Inhibits Oral Infection in STAT-1-/--Deficient Female Mice
    Gogal, R. M., Jr.
    Kerr, R.
    Kingsley, D. H.
    Granata, L. A.
    LeRoith, T.
    Holliman, S. D.
    Dancho, B. A.
    Flick, G. J., Jr.
    [J]. JOURNAL OF FOOD PROTECTION, 2011, 74 (02) : 209 - 214