Plasmonic ELISA for the detection of analytes at ultralow concentrations with the naked eye

被引：124

作者：

de la Rica, Roberto ^{[1
,2
,3
]}

Stevens, Molly M. ^{[1
,2
,3
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Mat, London, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Bioengn, London, England

[3] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London, England

来源：

NATURE PROTOCOLS | 2013年 / 8卷 / 09期

基金：

英国工程与自然科学研究理事会; 欧洲研究理事会;

关键词：

RAPID DETECTION;

D O I：

10.1038/nprot.2013.085

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This protocol describes a signal-generation mechanism for the naked-eye detection of analytes at low concentrations with ELISA. The key step is to generate solutions of desired tonality by growing gold nanoparticles with a particular state of aggregation. This is accomplished by linking the growth of gold nanoparticles with the biocatalytic cycle of the enzyme label. The protocol adapts a conventional ELISA procedure with catalase-labeled antibodies. The enzyme consumes hydrogen peroxide, and then gold (III) ions are added to generate gold nanoparticles. The concentration of hydrogen peroxide dictates the state of aggregation of gold nanoparticles. This allows for the naked-eye detection of analytes by observing the generation of blue- or red-colored gold nanoparticle solutions. When coupled with conventional ELISA, this signal-generation procedure allows for the naked-eye detection of analytes within 1 h.

引用

页码：1759 / 1764

页数：6

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