A ratiometric emission NIR-fluorescent probe for sensing and imaging pH changes in live cells

被引：28

作者：

Niu, Weifen ^{[1
,2
]}

Wei, Zhiwen ^{[1
]}

Jia, Juan ^{[1
]}

Shuang, Shaomin ^{[2
]}

Dong, Chuan ^{[2
]}

Yun, Keming ^{[1
]}

机构：

[1] Shanxi Med Univ, Sch Forens Med, Taiyuan 030001, Shanxi, Peoples R China

[2] Shanxi Univ, Coll Chem & Chem Engn, Inst Environm Sci, Taiyuan 030006, Shanxi, Peoples R China

来源：

DYES AND PIGMENTS | 2018年 / 152卷

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

pH; Ratiometric; NIR-fluorescent probe; Imaging; Live cells; STYRYLCYANINE;

D O I：

10.1016/j.dyepig.2018.01.050

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel ratiometric emission MR-fluorescence probe N,N-dimethyl-4-((1E, 3E)-4-(3,3-dimethyl-3H-indol-2-yl) buta-1,3-dienyl)benzenamine, namely, DIDBA, is facilely synthesized on the basis of ethylene bridging of indole derivatives and 4-(dimethylamino) cinnamaldehyde. The probe exhibits a remarkable NIR ratiometric fluorescence emission (F-618nm/F-697nm) characteristic with pK(a) 4.5, and the linear response over the pH range of 3.5-5.2. DIDBA also displays favorable features include large Stokes shift of 200 nm under acidic conditions, high sensitivity, good selectivity, fine photostability and reversibility. The probe has low cytotoxicity and excellent cell membrane permeability, and is applied successfully to visualize intracellular pH fluctuations in live cells without influence of autofluorescence and native cellular species in biological systems.

引用

页码：155 / 160

页数：6

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