Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses

被引：14

作者：

Zhu, Jiayi ^{[1
,2
]}

Yang, Qing ^{[3
]}

Cao, Liyan ^{[1
]}

Dou, Xiujing ^{[1
]}

Zhao, Jianguo ^{[4
]}

Zhu, Weijuan ^{[1
]}

Ding, Fan ^{[1
,5
]}

Bu, Ri-e ^{[2
]}

Suo, Siqingaowa ^{[1
]}

Ren, Yudong ^{[1
]}

Li, Guangxing ^{[1
]}

Ren, Xiaofeng ^{[1
,2
]}

机构：

[1] Northeast Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China

[2] Inner Mongolia Univ Nationalities, Sch Life Sci, Tongliao, Inner Mongolia, Peoples R China

[3] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China

[4] Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China

[5] Chinese Armed Police Forest Force Command, Dept Hlth, Beijing 100097, Peoples R China

来源：

VIROLOGY JOURNAL | 2013年 / 10卷

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

PRV; VP6; gene; Cloning; Expression; IDENTIFICATION;

D O I：

10.1186/1743-422X-10-91

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: The context and purpose of the study included 1) bacterial expression of viral protein 6 (VP6) of porcine rotavirus (PRV) and generation of rabbit polyclonal antiserum to the VP6 protein; 3) establishment of a discrimination ELISA to distinguish PRV from a panel of other porcine viruses. Results: The VP6 gene of PRV isolate DN30209 amplified by reverse transcription-PCR was 1356 bp containing a complete open reading frame (ORF) encoding 397 amino acids. Sequence comparison and phylogenetic analysis indicated that PRV DN30209 may belong to group A of rotavirus. Bacterially expressed VP6 was expressed in E. coli and anti-VP6 antibody was capable of distinguishing PRV from Porcine transmissible gastroenteritis virus, Porcine epidemic diarrhea virus, Porcine circovirus type II, Porcine reproductive and respiratory syndrome virus, Porcine pseudorabies virus and Porcine parvovirus. Conclusions: PRV VP6 expressed in E. coli can be used to generate antibodies in rabbit; anti-VP6 serum antibody can be used as good diagnostic reagents for detection of PRV.

引用

页数：8

共 28 条

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