Effects of zearalenone on calcium homeostasis of splenic lymphocytes of chickens in vitro

被引：10

作者：

Wang, Y. C. ^{[1
]}

Deng, J. L. ^{[1
]}

Xu, S. W. ^{[2
]}

Peng, X. ^{[3
]}

Zuo, Z. C.

Cui, H. M. ^{[3
]}

Wang, Y. ^{[4
]}

Ren, Z. H. ^{[4
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Yaan 625014, Sichuan, Peoples R China

[2] NE Agr Univ, Dept Vet Med, Harbin 150030, Peoples R China

[3] Sichuan Key Lab Anim Dis & Human Hlth, Yaan 625014, Sichuan, Peoples R China

[4] Sichuan Key Lab Environm Hazards Dis, Yaan 625014, Sichuan, Peoples R China

来源：

POULTRY SCIENCE | 2012年 / 91卷 / 08期

基金：

中国国家自然科学基金;

关键词：

zearalenone; calcium homeostasis; ATPase; calmodulin; mycotoxin; REAL-TIME PCR; INTRACELLULAR PH; SMOOTH-MUSCLE; OXIDATIVE STRESS; HYDROGEN-PEROXIDE; CALMODULIN; CYTOTOXICITY; CA2+; CHANNEL; CELLS;

D O I：

10.3382/ps.2011-02128

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Zearalenone (ZEA) is an estrogenic mycotoxin. It is produced by several Fusarium species and can contaminate food and feed. To investigate the role of calcium homeostasis in ZEA-induced toxicity of poultry and elucidate its cytotoxic mechanism, splenic lymphocytes isolated from chickens were exposed to ZEA (0-25 mu g/mL) for 48 h. The intracellular calcium concentration ([Ca2+]i), pH, calmodulin (CaM) mRNA levels, and Na+/K+-ATPase activities and Ca(2+-)ATPase activities were detected by the fluorescent dyes Fluo-3/AM and BCECF/AM, quantitative real-time PCR, and chromatometry. Supernatant CaM concentrations were simultaneously detected by ELISA. As the ZEA exposure concentration increased, the [Ca2+] i and CaM mRNA levels gradually increased, while intracellular pH, CaM concentrations of supernatants, and intracellular Na+/K+-ATPase and Ca2+-ATPase activities gradually decreased in a dose-dependent manner. There were significant differences (P < 0.05 or P < 0.01) between the treatment groups and the control group. These results indicate that ZEA cytotoxicity arises by causing an imbalance in calcium homeostasis and intracellular acidification in lymphocytes.

引用

页码：1956 / 1963

页数：8

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