ProteinRNA Interfaces Probed by 1H-Detected MAS Solid-State NMR Spectroscopy

被引：49

作者：

Asami, Sam ^{[1
,2
]}

Rakwalska-Bange, Magdalena ^{[3
]}

Carlomagno, Teresa ^{[3
]}

Reif, Bernd ^{[1
,2
]}

机构：

[1] TUM, Dept Chem, CIPS M, D-85747 Garching, Germany

[2] Helmholtz Zentrum Munchen HMGU, Deutsch Forschungszentrum Gesundheit & Umwelt, D-85764 Neuherberg, Germany

[3] EMBL, Computat & Struct Biol Unit, D-69117 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 08期

关键词：

NMR spectroscopy; proteins; RNA; proton detection; structural restraints; BOX C/D RNA; HIGH-RESOLUTION; RIBOSE METHYLATION; ASSIGNMENT; SEQUENCE; FIBRILS; PROGRAM; COMPLEX;

D O I：

10.1002/anie.201208024

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Both protonated and deuterated samples were employed in the study of the L7Ae box C/D RNA complex by 1H-detected solid-state NMR spectroscopy. This approach yielded high-resolution spectra and was used to determine the intermolecular interface and extract structural parameters with high accuracy. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：2345 / 2349

页数：5

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