Optimization SNEDDS (Self-Nano Emulsifying Drug Delivery System) of ZnO that dispersed into Hydrogel Matrix as UV-Protective

World Health Organization in 2015, estimated the case of non-melanoma cancer increasing due to the depletion of the ozone layer. The case of skin cancer in Indonesia reaches 6-8%, so the skin needs effective protection. ZnO is a sunscreen with SPF [Sun Protecting Factor] value of 50 and sun exposure reduction to the skin, especially UV A [320-400 nm] and UV B [290-320 nm]. ZnO is difficult to dissolve in water and hard to dispersed in the hydrogel matrix, so that it is formulated a nanoemulsion preparation into SNEDDS. SNEDDS consists of olive oil, tween 80 [surfactant], and propylene glycol [cosurfactant]. Optimization of the SNEDDS ZnO with the D-Optimal method was using Design Expert Software producing 16 formulas that tested for physical stability response including transmittance values [%] and pH values. The formula that has the highest response value with the desirability value approaching one was the optimum formula, then analyzed done with PSA. The optimum SNEDDS ZnO was dispersed in the hydrogel matrix of carbopol, thentest included pH, viscosity, dispersion and SPF value. The optimization shows that the optimum composition of olive oil : tween 80 : propylene glycol of 1:9:1. The result of t-test of SNEDDS ZnO optimum formula were not significantly different (p-value> 0,05) with Design Expert prediction. Optimum formula can load 2 mg-ZnO with transmitance value is 92%, SPF value 26, PSA 150 nm, and polydispers size of droplets.