Neuroprotective Effects of Direct Intrathecal Administration of Granulocyte Colony-Stimulating Factor in Rats with Spinal Cord Injury

被引：42
作者：
Chen, Wu-Fu [1 ,2 ,3 ,4 ]
Chen, Chun-Hong [5 ,6 ]
Chen, Nan-Fu [7 ]
Sung, Chun-Sung [8 ,9 ]
Wen, Zhi-Hong [4 ,5 ,6 ]
机构：
[1] Kaohsiung Chang Gung Mem Hosp, Dept Neurosurg, Kaohsiung, Taiwan
[2] Chang Gung Univ, Coll Med, Kaohsiung, Taiwan
[3] Xiamen Chang Gung Mem Hosp, Dept Neurosurg, Xiamen, Peoples R China
[4] Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Kaohsiung 80424, Taiwan
[5] Natl Sun Yat Sen Univ, Doctoral Degree Program Marine Biotechnol, Kaohsiung 80424, Taiwan
[6] Acad Sinica, Kaohsiung, Taiwan
[7] Kaohsiung Armed Forces Gen Hosp, Dept Surg, Div Neurosurg, Kaohsiung, Taiwan
[8] Taipei Vet Gen Hosp, Dept Anesthesiol, Taipei, Taiwan
[9] Natl Yang Ming Univ, Sch Med, Taipei 112, Taiwan
来源：
CNS NEUROSCIENCE & THERAPEUTICS | 2015年 / 21卷 / 09期
关键词：
G-CSF; Intrathecal; Methylprednisolone; Spinal cord injury; Transforming growth factor; CHONDROITIN SULFATE PROTEOGLYCANS; GROWTH-FACTOR-BETA; FOCAL CEREBRAL-ISCHEMIA; CENTRAL-NERVOUS-SYSTEM; CNS GLIAL SCAR; TRANSFORMING GROWTH-FACTOR-BETA-1; MESSENGER-RNA; REACTIVE ASTROCYTES; SIGNALING PATHWAY; CONTROLLED-TRIAL;
D O I：
10.1111/cns.12429
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
AimsTo date, no reliable methods have proven effective for treating spinal cord injury (SCI). Even systemic administration of methylprednisolone (MP) remains controversial. We previously reported that intrathecal (i.t.) administration of granulocyte colony-stimulating factor (G-CSF) improves outcome after experimental spinal cord ischemic insults in rats. The present study aimed to examine the neuroprotective efficacy of i.t. G-CSF or MP in rats with SCI. MethodsFemale rats were subjected to spinal cord contusion injury at T10 using NYU impactor. We i.t. administered G-CSF (10g) or MP (one bolus of 100g, followed by 18g/h infusion for 23h) immediately after SCI. ResultsBoth G-CSF and MP significantly improved the rats' motor function after SCI. Immunofluorescence staining revealed suppressed expression of transforming growth factor-beta 1 (TGF-1), chondroitin sulfate proteoglycans (neurocan and phosphacan), OX-42 and tumor necrosis factor alpha after i.t. G-CSF, but not MP, in rats with SCI. In addition, G-CSF significantly decreased the expression of astrocytic TGF-1 and glial fibrillary acidic protein around the injury site. Furthermore, rats with G-CSF treatment showed increased neurofilament expression beyond the glial scars. ConclusionDirect i.t. administration of G-CSF provides a promising therapeutic option for SCI or related spinal diseases.
引用
收藏
页码：698 / 707
页数：10
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