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Preserved Ex Vivo Inflammatory Status in Decidual Cells from Women with Preterm Labor and Subclinical Intrauterine Infection
被引:14
作者:
Castro-Leyva, Violeta
[1
,2
]
Espejel-Nunez, Aurora
[3
]
Barroso, Gerardo
[4
]
Zaga-Clavellina, Veronica
[5
]
Flores-Pliego, Arturo
[3
]
Morales-Mendez, Iyari
[1
]
Giono-Cerezo, Silvia
[2
]
Walsh, Scott W.
[6
]
Estrada-Gutierrez, Guadalupe
[1
]
机构:
[1] Inst Nacl Perinatol Isidro Espinosa de los Reyes, Dept Infectol, Mexico City, DF, Mexico
[2] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico
[3] Inst Nacl Perinatol Isidro Espinosa de los Reyes, Dept Biochem & Mol Biol, Mexico City, DF, Mexico
[4] Inst Nacl Perinatol Isidro Espinosa de los Reyes, Div Clin Res, Mexico City, DF, Mexico
[5] Inst Nacl Perinatol Isidro Espinosa de los Reyes, Dept Cellular Biol, Mexico City, DF, Mexico
[6] Virginia Commonwealth Univ, Dept Obstet & Gynecol & Physiol & Biophys, Med Ctr, Richmond, VA USA
来源:
关键词:
NECROSIS-FACTOR-ALPHA;
CERVICAL-MUCUS PLUG;
MATRIX METALLOPROTEINASES;
PROSTAGLANDIN BIOSYNTHESIS;
AMNIOTIC-FLUID;
BIRTH;
PARTURITION;
CYTOKINES;
MEMBRANES;
LIPOPOLYSACCHARIDE;
D O I:
10.1371/journal.pone.0043605
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Objective: To compare the inflammatory response preserved ex vivo by decidual cells isolated from women who experienced preterm labor with and without subclinical intrauterine infection. Methods: Fetal membranes were obtained after cesarean section from 35 women who delivered before 37 weeks of gestation following spontaneous preterm labor, with no clinical evidence of intrauterine infection. Decidua was microbiologically tested and cultured. Concentrations of anti-inflammatory cytokines (IL-2, IL-4, IL-10), pro-inflammatory cytokines (IL-6, IL-8, IL-1 beta and TNF-alpha), and matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9) were measured in the supernatants using Bio-Plex, and prostaglandin E-2 (PGE(2)) was measured by enzyme immunoassay. Results: Subclinical infection was confirmed in 10 women (28.5%). Microorganisms isolated were Ureaplasma urealyticum (4), group B streptococci (3), Gardnerella vaginalis (1), and Escherichia coli (2). We found a significant increase of proinflammatory cytokines and a significant decrease of anti-inflammatory cytokines in supernatants from decidual cells obtained from women with preterm labor and subclinical intrauterine infection compared to women without infection. Secretion of MMP-1, MMP-8, MMP-9 and PGE(2) was significantly higher in infected women. Secretion of IL-8 by decidual cells from infected women persisted upon repeated in vitro culture passages. Conclusions: Almost 30% of idiopathic preterm labor cases were associated with subclinical intrauterine infection, and decidual cells isolated from these cases preserved an ex vivo inflammatory status after in vivo bacterial exposure.
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