Preserved Ex Vivo Inflammatory Status in Decidual Cells from Women with Preterm Labor and Subclinical Intrauterine Infection

被引:14
作者
Castro-Leyva, Violeta [1 ,2 ]
Espejel-Nunez, Aurora [3 ]
Barroso, Gerardo [4 ]
Zaga-Clavellina, Veronica [5 ]
Flores-Pliego, Arturo [3 ]
Morales-Mendez, Iyari [1 ]
Giono-Cerezo, Silvia [2 ]
Walsh, Scott W. [6 ]
Estrada-Gutierrez, Guadalupe [1 ]
机构
[1] Inst Nacl Perinatol Isidro Espinosa de los Reyes, Dept Infectol, Mexico City, DF, Mexico
[2] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico
[3] Inst Nacl Perinatol Isidro Espinosa de los Reyes, Dept Biochem & Mol Biol, Mexico City, DF, Mexico
[4] Inst Nacl Perinatol Isidro Espinosa de los Reyes, Div Clin Res, Mexico City, DF, Mexico
[5] Inst Nacl Perinatol Isidro Espinosa de los Reyes, Dept Cellular Biol, Mexico City, DF, Mexico
[6] Virginia Commonwealth Univ, Dept Obstet & Gynecol & Physiol & Biophys, Med Ctr, Richmond, VA USA
关键词
NECROSIS-FACTOR-ALPHA; CERVICAL-MUCUS PLUG; MATRIX METALLOPROTEINASES; PROSTAGLANDIN BIOSYNTHESIS; AMNIOTIC-FLUID; BIRTH; PARTURITION; CYTOKINES; MEMBRANES; LIPOPOLYSACCHARIDE;
D O I
10.1371/journal.pone.0043605
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Objective: To compare the inflammatory response preserved ex vivo by decidual cells isolated from women who experienced preterm labor with and without subclinical intrauterine infection. Methods: Fetal membranes were obtained after cesarean section from 35 women who delivered before 37 weeks of gestation following spontaneous preterm labor, with no clinical evidence of intrauterine infection. Decidua was microbiologically tested and cultured. Concentrations of anti-inflammatory cytokines (IL-2, IL-4, IL-10), pro-inflammatory cytokines (IL-6, IL-8, IL-1 beta and TNF-alpha), and matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9) were measured in the supernatants using Bio-Plex, and prostaglandin E-2 (PGE(2)) was measured by enzyme immunoassay. Results: Subclinical infection was confirmed in 10 women (28.5%). Microorganisms isolated were Ureaplasma urealyticum (4), group B streptococci (3), Gardnerella vaginalis (1), and Escherichia coli (2). We found a significant increase of proinflammatory cytokines and a significant decrease of anti-inflammatory cytokines in supernatants from decidual cells obtained from women with preterm labor and subclinical intrauterine infection compared to women without infection. Secretion of MMP-1, MMP-8, MMP-9 and PGE(2) was significantly higher in infected women. Secretion of IL-8 by decidual cells from infected women persisted upon repeated in vitro culture passages. Conclusions: Almost 30% of idiopathic preterm labor cases were associated with subclinical intrauterine infection, and decidual cells isolated from these cases preserved an ex vivo inflammatory status after in vivo bacterial exposure.
引用
收藏
页数:6
相关论文
共 31 条
[1]   Production of matrix metalloproteinase-9 in lipopolysaccharide-stimulated human amnion occurs through an autocrine and paracrine proinflammatory cytokine-dependent system [J].
Arechavaleta-Velasco, F ;
Ogando, D ;
Parry, S ;
Vadillo-Ortega, F .
BIOLOGY OF REPRODUCTION, 2002, 67 (06) :1952-1958
[2]   Matrix metalloproteinases in the cervical mucus plug in relation to gestational age, plug compartment, and preterm labor [J].
Becher, Naja ;
Hein, Merete ;
Danielsen, Carl C. ;
Uldbjerg, Niels .
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2010, 8
[3]   Balance between matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) in the cervical mucus plug estimated by determination of free non-complexed TIMP [J].
Becher, Naja ;
Hein, Merete ;
Uldbjerg, Niels ;
Danielsen, Carl Christian .
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2008, 6 (1)
[4]   The worldwide incidence of preterm birth: a systematic review of maternal mortality and morbidity [J].
Beck, Stacy ;
Wojdyla, Daniel ;
Say, Lale ;
Betran, Ana Pilar ;
Merialdi, Mario ;
Requejo, Jennifer Harris ;
Rubens, Craig ;
Menon, Ramkumar ;
Van Look, Paul F. A. .
BULLETIN OF THE WORLD HEALTH ORGANIZATION, 2010, 88 (01) :31-38
[5]   Cytokines of the placenta and extra-placental membranes: Roles and regulation during human pregnancy and parturition [J].
Bowen, JM ;
Chamley, L ;
Keelan, JA ;
Mitchell, MD .
PLACENTA, 2002, 23 (04) :257-273
[6]   Microbial Prevalence, Diversity and Abundance in Amniotic Fluid During Preterm Labor: A Molecular and Culture-Based Investigation [J].
DiGiulio, Daniel B. ;
Romero, Roberto ;
Amogan, Harold P. ;
Kusanovic, Juan Pedro ;
Bik, Elisabeth M. ;
Gotsch, Francesca ;
Kim, Chong Jai ;
Erez, Offer ;
Edwin, Sam ;
Relman, David A. .
PLOS ONE, 2008, 3 (08)
[7]   BIOSYNTHESIS OF INTERLEUKIN-6 BY CULTURED HUMAN CHORION LAEVE CELLS - REGULATION BY CYTOKINES [J].
DUDLEY, DJ ;
TRAUTMAN, MS ;
EDWIN, SS ;
LUNDINSCHILLER, S ;
MITCHELL, MD .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1992, 75 (04) :1081-1086
[8]   Initial characterization of the microenvironment that regulates connective tissue degradation in amniochorion during normal human labor [J].
Estrada-Gutierrez, G ;
Zaga, V ;
Gonzalez-Jimenez, MA ;
Beltran-Montoya, J ;
Maida-Claros, R ;
Giono-Cerezo, S ;
Vadillo-Ortega, F .
MATRIX BIOLOGY, 2005, 24 (04) :306-312
[9]   Interaction between Pathogenic Bacteria and Intrauterine Leukocytes Triggers Alternative Molecular Signaling Cascades Leading to Labor in Women [J].
Estrada-Gutierrez, Guadalupe ;
Gomez-Lopez, Nardhy ;
Zaga-Clavellina, Veronica ;
Giono-Cerezo, Silvia ;
Espejel-Nunez, Aurora ;
Antonio Gonzalez-Jimenez, Marco ;
Espino y Sosa, Salvador ;
Olson, David M. ;
Vadillo-Ortega, Felipe .
INFECTION AND IMMUNITY, 2010, 78 (11) :4792-4799
[10]   Evidence of in vitro differential secretion of 72 and 92 kDa type IV collagenases after selective exposure to lipopolysaccharide in human fetal membranes [J].
Garcia-Lopez, Guadalupe ;
Vadillo-Ortega, Felipe ;
Merchant-Larios, Horacio ;
Maida-Claros, Rolando ;
Osorio, Mauricio ;
Soriano-Becerril, Diana ;
Flores-Herrera, Hector ;
Beltran-Montoya, Jorge ;
Garfias-Becerra, Yonathan ;
Zaga-Clavellina, Verónica .
MOLECULAR HUMAN REPRODUCTION, 2007, 13 (5-6) :409-418