Generation of large insert yeast artificial chromosome libraries

被引：3

作者：

Larin, Z ^{[1
]}

Monaco, AP ^{[1
]}

Lehrach, H ^{[1
]}

机构：

[1] UNIV OXFORD,JOHN RADCLIFFE HOSP,INST MOL MED,OXFORD OX3 9DS,ENGLAND

来源：

MOLECULAR BIOTECHNOLOGY | 1997年 / 8卷 / 02期

关键词：

YAC; PFGE; polyamines;

D O I：

10.1007/BF02752259

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of YAC cloning technology has directly enhanced the relationship among genetic, physical, and functional mapping of genomes. Because of their large size, YACs have enabled the rapid construction of physical maps by ordered clone mapping and contig building, and they complement other molecular approaches for mapping complex genomes. Large insert libraries are constructed by size fractionating large DNA embedded in agarose and protecting DNA from degradation with polyamines.

引用

页码：147 / 153

页数：7

共 27 条

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