Widespread Distribution of Soluble Di-Iron Monooxygenase (SDIMO) Genes in Arctic Groundwater Impacted by 1,4-Dioxane

被引:51
作者
Li, Mengyan [1 ]
Mathieu, Jacques [1 ]
Yang, Yu [1 ]
Fiorenza, Stephanie [2 ]
Deng, Ye [3 ]
He, Zhili [3 ]
Zhou, Jizhong [4 ,5 ]
Alvarez, Pedro J. J. [1 ]
机构
[1] Rice Univ, Dept Civil & Environm Engn, Houston, TX 77251 USA
[2] BP Amer, Remediat Engn & Technol, Houston, TX USA
[3] Univ Oklahoma, Inst Environm Genom, Dept Microbiol & Plant Biol, Norman, OK 73019 USA
[4] Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China
[5] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
关键词
SP STRAIN; MICROBIAL COMMUNITIES; BIODEGRADATION; DEGRADATION; DIVERSITY; GEOCHIP; TETRAHYDROFURAN; IDENTIFICATION; EXPRESSION; EVOLUTION;
D O I
10.1021/es402228x
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Soluble di-iron monooxygenases (SDIMOs), especially group-5 SDIMOs (i.e., tetrahydrofuran and propane monooxygenases), are of significant interest due to their potential role in the initiation of 1,4-dioxane (dioxane) degradation. Functional gene array (i.e., GeoChip) analysis of Arctic groundwater exposed to dioxane since 1980s revealed that various dioxane-degrading SDIMO genes were widespread, and PCR-DGGE analysis showed that group-5 SDIMOs were present in every tested sample, including background groundwater with no known dioxane exposure history. A group-5 thmA-like gene was enriched (2.4-fold over background, p < 0.05) in source-zone samples with higher dioxane concentrations, suggesting selective pressure by dioxane. Microcosm assays with C-14-labeled dioxane showed that the highest mineralization capacity (6.4 +/- 0.1% (CO2)-C-14 recovery during 15 days, representing over 60% of the amount degraded) corresponded to the source area, which was presumably more acclimated and contained a higher abundance of SDIMO genes. Dioxane mineralization ceased after 7 days and was resumed by adding acetate (0.24 mM) as an auxiliary substrate to replenish NADH, a key coenzyme for the functioning of monoxygenases. Acetylene inactivation tests further corroborated the vital role of monooxygenases in dioxane degradation. This is the first report of the prevalence of oxygenase genes that are likely involved in dioxane degradation and suggests their usefulness as biomarkers of dioxane natural attenuation.
引用
收藏
页码:9950 / 9958
页数:9
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