A preliminary study on the detection of lymph node metastasis in cervical cancer using a quantitative RT-PCR assay

被引：2

作者：

Togami, Shinichi ^{[1
]}

Fukuda, Mika ^{[1
]}

Yanazume, Shintaro ^{[1
]}

Kamio, Masaki ^{[1
]}

Kobayashi, Hiroaki ^{[1
]}

机构：

[1] Kagoshima Univ, Fac Med, Dept Obstet & Gynecol, Sakuragaoka 8-35-1, Kagoshima 8908520, Japan

来源：

JAPANESE JOURNAL OF CLINICAL ONCOLOGY | 2022年 / 52卷 / 05期

关键词：

cervical cancer; cytokeratin; 19; mRNA; sentinel lymph node; BREAST-CANCER; MARKER;

D O I：

10.1093/jjco/hyac006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The quantitative reverse transcriptase-polymerase chain reaction assay using cytokeratin 19 mRNA may be an alternative to the conventional pathological lymph node diagnosis of cervical cancer. Objective This preliminary study aimed to assess the detection accuracy of sentinel lymph node metastasis in cervical cancer using quantitative reverse transcriptase-polymerase chain reaction. Methods We collected cervical cancer tissues and 70 pelvic lymph node samples from patients with cervical cancer. The quantitative reverse transcriptase-polymerase chain reaction assay was performed to investigate the expression of cytokeratin 19 mRNA in cervical cancer tissues and determine the cutoff value of cytokeratin 19 mRNA between the non-metastatic and metastatic lymph nodes. Results The expression of cytokeratin 19 mRNA in cancer tissues was detected in all (71/71) the tumours, with a median copy number of 7.56 x 10(5)/mu l of RNA by quantitative reverse transcriptase-polymerase chain reaction. Sixteen lymph nodes were diagnosed as positive by pathological examination. The median copy numbers of cytokeratin 19 mRNA for positive and negative lymph nodes were 43.3 x 10(4)/mu l and 121.1/mu l, respectively. The expression of cytokeratin 19 mRNA in pathologically positive lymph nodes was higher than that in the negative lymph nodes (P < 0.0001) by quantitative reverse transcriptase-polymerase chain reaction analysis. Using a receiver operating characteristic plot, the maximum sensitivity (100%) and specificity (94.4%) were obtained when the cutoff value was set at 1169 copies/mu l. Conclusions After setting the cutoff value at 1169 copies/mu l, a quantitative reverse transcriptase-polymerase chain reaction assay using cytokeratin 19 mRNA showed high accuracy in detecting lymph node metastasis in cervical cancer. We believe that the quantitative reverse transcriptase-polymerase chain reaction assay using cytokeratin 19 mRNA may be acceptable for lymph node metastasis detection in patients with cervical cancer.

引用

页码：475 / 478

页数：4

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