Activation of the Akt pathway by a constitutive androstane receptor agonist results in β-catenin activation

It is well known that activating the constitutive androstane receptor (CAR, NR1I3) leads to a significant proliferation of liver cells, which suggests that NR1I3 could be a therapeutic target for the partial resection of this organ. Studies describing NR1I3-mediated proliferative pathways could help determine the possible clinical applications of NR1I3 agonists during liver resection or transplantation. Recently, we reported that liver hyperplasia, which results from NR1I3 activation, is mediated by the activation of the Akt signaling pathway. In this study, we investigated the impact of the Akt signaling pathway on beta-catenin and its role in liver growth. Our findings showed that NR1I3-mediated activation of the Akt pathway results in the nuclear redistribution of beta-catenin and increases hepatocyte proliferation. Inhibiting the Akt pathway using the allosteric inhibitor MK2206 decreased the amount of beta-catenin in the nucleus and reduced the hepatocyte proliferation induced by a NR1I3 agonist, but promoted hypertrophic liver growth. These findings suggest that NR1I3-mediated hepatocyte proliferation and liver growth are not necessarily linked. Additionally, we found that the proliferation effect of the NR1I3-Akt-beta-catenin signaling pathway is mediated, at least in part, by Cyclic D1 up-regulation. In contrast, the nuclear localization of beta-catenin in response to NR1I3 did not affect the expression of the beta-catenin target cMyc in the liver. In conclusion, the NR1I3-Akt signaling pathway plays a significant role in regulating hepatocyte proliferation, at least in part, by activating beta-catenin.