Overexpression of IQGAP1 in human pancreatic cancer

被引:29
作者
Wang, Xiao-Xia [1 ,4 ]
Li, Xiao-Zhong [2 ]
Zhai, Li-Qin [3 ]
Liu, Zhi-Rong [1 ]
Chen, Xian-Jiu [1 ]
Pei, Yi [4 ]
机构
[1] Shanxi Med Univ, Dept Biochem & Mol Biol, Taiyuan 030001, Peoples R China
[2] Shanxi Prov Peoples Hosp, Dept Emergency, Taiyuan 030012, Peoples R China
[3] Shanxi Prov Peoples Hosp, Dept Pathol, Taiyuan 030012, Peoples R China
[4] Shanxi Dayi Hosp, Shanxi Acad Med Sci, Dept Geriatr Oncol, Taiyuan 030032, Peoples R China
基金
中国国家自然科学基金;
关键词
IQGAP1; pancreatic cancer; tissue microarray; immunohistochemistry; E-CADHERIN; EXPRESSION; CARCINOMA; RAC1; CDC42; PROLIFERATION; STIMULATION; PARAMETERS; MUTATIONS; PROGNOSIS;
D O I
10.1016/S1499-3872(13)60085-5
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
BACKGROUND: Pancreatic cancer is a highly aggressive malignant tumor with the lowest survival rate. A better understanding of the molecular mechanisms which contribute to pancreatic cancer occurrence and progression will aid in the development of new approaches to the early diagnosis, prevention, and treatment of this deadly disease. The scaffold protein IQGAP1 shows elevated levels in a variety of cancer types. Currently, we investigated whether or not IQGAP1 is also overexpressed in pancreatic cancer. METHODS: IQGAP1 expression was examined in pancreatic cancer and normal tissues adjacent to cancerous tissues (adjacent tissues) by Western blotting and real-time RT-PCR as well as in paraffin sections of tissue microarray. by immunohistochemistry. The correlations between IQGAP1 expression and various clinicopathological characteristics were analyzed. RESULTS: Western blotting and real-time RT-PCR revealed that the levels of IQGAP1 protein and mRNA expression in pancreatic cancer tissues were significantly increased compared with adjacent tissues. Immunohistochemistry analysis on tissue microarray showed that IQGAP1 protein expression was significantly higher in pancreatic cancer (80.0%, 48/60) compared with adjacent tissues (18.3%, 11/60) (P<0.001). Moreover, overexpression of IQGAP1 was shown to be associated with the grades of tumor differentiation (P<0.05). CONCLUSION: The overexpression of IQGAP1 may play an important role in pancreatic cancer occurrence and progression, and IQGAP1 may serve as a novel molecular target for the diagnosis and treatment of pancreatic cancer.
引用
收藏
页码:540 / 545
页数:6
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