Establishment of in vitro culture, plant regeneration, and genetic transformation of Camelina sativa

被引：17
|
作者：
Yemets, A. I. [1 ]
Boychuk, Yu. N. [1 ]
Shysha, E. N. [1 ]
Rakhmetov, D. B. [2 ]
Blume, Ya. B. [1 ]
机构：
[1] Natl Acad Sci Ukraine, Inst Food Biotechnol & Genom, Kiev, Ukraine
[2] Natl Acad Sci Ukraine, Gryshko Natl Bot Garden, Kiev, Ukraine
来源：
CYTOLOGY AND GENETICS | 2013年 / 47卷 / 03期
关键词：
SHOOT REGENERATION; EMBRYOGENESIS; FUSION; L;
D O I：
10.3103/S0095452713030031
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The results of establishing an in vitro culture, plantlet regeneration, and rooting of Camelina sativa cultivar Peremozhets and cultivar Mirazh are presented. The effective concentrations of sterilizing agents and the duration of plant material treatment were estimated. The phytohormone ratio, the sucrose concentration in the nutrient medium that induced the effective formation of C. sativa shoots, and the NAA concentration for plantlet rooting have been established. A method of Agrobacterium-mediated transformation of Camelina by using binary vector pGH217 carrying the reporter beta-glucoronidase (gus) gene driven under the 35S CaMV promoter and nos-terminator, as well as the selective marker hpt gene conferring hygromycin-resistance in transgenic plant, was elaborated.
引用
收藏
页码：138 / 144
页数：7
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