Fast estimation of adsorption isotherm parameters in gradient elution preparative liquid chromatography. I: The single component case

被引:24
作者
Asberg, Dennis [1 ]
Lesko, Marek [2 ]
Enmark, Martin [1 ]
Samuelsson, Jorgen [1 ]
Kaczmarski, Krzysztof [2 ]
Fornstedt, Torgny [1 ]
机构
[1] Karlstad Univ, Dept Engn & Chem Sci, SE-65188 Karlstad, Sweden
[2] Rzeszow Univ Technol, Dept Chem Engn, PL-35959 Rzeszow, Poland
基金
瑞典研究理事会;
关键词
RPLC; Gradient elution; Inverse method; Adsorption isotherm determination; REVERSED-PHASE SYSTEMS; ENERGY-DISTRIBUTIONS; NUMERICAL ESTIMATION; PROTEIN SAMPLES; BAND PROFILE; PERFORMANCE; MULTICOMPONENT; OPTIMIZATION; ENANTIOMERS; SEPARATION;
D O I
10.1016/j.chroma.2013.05.041
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The inverse method is a numeric method for fast estimation of adsorption isotherm parameters directly from overloaded elution profiles. However, it has previously only been used for isocratic experiments. Here we will extend the inverse method so it can be used for gradient elution too. This extended inverse method will make it possible to study the adsorption of substances whose retention factor vary strongly with the mobile-phase composition, like peptides and proteins, where the classic methods will fail. Our extended inverse method was verified using both simulations and real experiments. For simulated overloaded elution profiles we were able to determine almost exact Langmuir adsorption isotherm parameters with the new approach. From real experimental data, bi-Langmuir adsorption parameters were estimated using both the perturbation peak method and the extended inverse method. The shape of the acquired adsorption isotherms did match over the considered concentration range; however, the adsorption isotherm parameters found with the two methods were not the same. This is probably due to the fact that adsorption isotherm estimated with the inverse method is only a good approximation up to the highest eluted concentration in the used chromatograms. But this is not a serious drawback from a process point of view where the main objective is to make accurate predictions of elution profiles. The bi-Langmuir adsorption isotherm obtained with both methods could accurately predict the shape of overloaded elution profiles. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:64 / 70
页数:7
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