Development of a Monoclonal Antibody-Based ELISA for the Detection of Sulfaquinoxaline in Chicken Tissues and Serum

被引：9

作者：

Li, Xiangmei ^{[1
]}

Tang, Shusheng ^{[1
]}

Chen, Yiqiang ^{[1
]}

Niu, Lijuan ^{[1
]}

Ding, Shuangyang ^{[1
]}

Wan, Yongping ^{[2
]}

Xiao, Xilong ^{[1
]}

机构：

[1] China Agr Univ, Dept Pharmacol & Toxicol, Coll Vet Med, Beijing 100193, Peoples R China

[2] China Agr Univ, Natl Vet Drug Safety Evaluat Ctr, Beijing 100193, Peoples R China

来源：

ANALYTICAL LETTERS | 2008年 / 41卷 / 17期

关键词：

Chicken tissues; ELISA; monoclonal antibody; serum; sulfaquinoxaline;

D O I：

10.1080/00032710802463162

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A monoclonal antibody (Mab) was produced by using sulfaquinoxaline-human serum albumin (SQX-HSA) conjugate as immunogen. The anti-SQX Mab exhibited negligible cross reactivity with other commonly used sulfonamides. Using this Mab, a competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed to detect SQX in chicken tissues and serum. The ciELISA showed a 50% inhibition (IC50) value of 2.60ng/mL. The recoveries of SQX from spiked chicken muscle, liver, and serum at levels of 5-50g/kg were 82.6-96.5%, 75.3-94.5%, and 69.7-89.3%, respectively. The coefficient variations (CVs) were 6.22-7.17%, 4.9-8.9%, and 1.20-10.15%, respectively. Detection limits were 1.29g/kg in muscle, 1.32g/kg in liver, and 2.44g/kg in serum.

引用

页码：3007 / 3020

页数：14

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